Human CCL2/MCP-1 ELISA Kit - Quantikine Best Seller
R&D Systems, part of Bio-Techne | Catalog # DCP00
Key Product Details
Assay Length
3.5 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Citrate Plasma (100 µL), Urine (100 µL)
Sensitivity
10 pg/mL
Assay Range
31.2-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
Product Summary for Human CCL2/MCP-1 Quantikine ELISA Kit
The Quantikine Human MCP-1 Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure MCP-1 in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human MCP-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human MCP-1. Results obtained using natural human MCP-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MCP-1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human MCP-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma/Urine - Samples from apparently healthy volunteers were evaluated for the
presence of human MCP-1 in this assay. No medical histories were available for the donors used
in this study. The reported urine values are actual and are not normalized for creatinine
content.
Cell Culture Supernates - Human peripheral blood leukocytes were cultured in RPMI 1640 and supplemented with 10% fetal bovine serum. The cells were cultured unstimulated or stimulated with 10 μg/mL PHA for 2 or 5 days. Aliquots of the cell culture supernates were removed and assayed for levels of human MCP-1.
| Sample Type | Mean (pg/mL) | Range (pg/mL) |
| Serum (n=37) | 370 | 200-722 |
| EDTA plasma (n=37) | 153 | 72-295 |
| Citrate plasma (n=37) | 196 | 134-436 |
| Heparin plasma (n=37) | 242 | 113-340 |
| Urine (n=37) | 211 | 42-410 |
Cell Culture Supernates - Human peripheral blood leukocytes were cultured in RPMI 1640 and supplemented with 10% fetal bovine serum. The cells were cultured unstimulated or stimulated with 10 μg/mL PHA for 2 or 5 days. Aliquots of the cell culture supernates were removed and assayed for levels of human MCP-1.
| Condition | Day 2 (pg/mL) | Day 5 (pg/mL) |
| Unstimulated | 647 | 1785 |
| Stimulated | 67,225 | 70,000 |
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 78.2 | 360 | 1079 | 76.0 | 360 | 1086 |
| Standard Deviation | 3.3 | 17.7 | 63.5 | 4.5 | 17.2 | 48.5 |
| CV% | 4.2 | 4.9 | 5.9 | 5.9 | 4.8 | 4.5 |
Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 76.7 | 364 | 1121 | 74.2 | 352 | 1076 |
| Standard Deviation | 6.0 | 17.1 | 54.4 | 5.0 | 20.5 | 49.4 |
| CV% | 7.8 | 4.7 | 4.9 | 6.7 | 5.8 | 4.6 |
Recovery for Human CCL2/MCP-1 Quantikine ELISA Kit
The recovery of MCP-1 spiked to three levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 96 | 88-107 |
| Citrate Plasma (n=5) | 100 | 94-107 |
| EDTA Plasma (n=5) | 96 | 92-102 |
| Heparin Plasma (n=5) | 102 | 94-114 |
| Serum (n=5) | 103 | 92-113 |
| Urine (n=5) | 92 | 85-100 |
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of MCP-1 and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human CCL2/MCP-1 Quantikine ELISA Kit
Human MCP-1 ELISA/CCL2 ELISA Cell Culture Supernate/Urine Standard Curve
Human MCP-1 ELISA/CCL2 ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL2/JE/MCP-1
Alternate Names
GDCF-2, HC11, HSMCR30, MCAF, MCP-1, SMC-CF
Gene Symbol
CCL2
Additional CCL2/JE/MCP-1 Products
Product Documents for Human CCL2/MCP-1 Quantikine ELISA Kit
Product Specific Notices for Human CCL2/MCP-1 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.