Human CXCL11/I-TAC Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DCX110
Key Product Details
Assay Length
Sample Type & Volume Required Per Well
Sensitivity
Assay Range
Product Summary for Human CXCL11/I-TAC Quantikine ELISA Kit
Product Specifications
Assay Type
Format
Measurement
Detection Method
Conjugate
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean (pg/mL) | 353 | 1179 | 2191 | 409 | 1290 | 2401 |
Standard Deviation | 18 | 40.6 | 102.9 | 35.5 | 93 | 179 |
CV% | 5.1 | 3.4 | 4.7 | 8.7 | 7.2 | 7.5 |
EDTA Plasma, Heparin Plasma, Serum
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean (pg/mL) | 403 | 1321 | 2481 | 437 | 1403 | 2644 |
Standard Deviation | 19 | 62.5 | 147.3 | 35.1 | 107 | 190 |
CV% | 4.7 | 4.7 | 5.9 | 8.0 | 7.6 | 7.2 |
Recovery for Human CXCL11/I-TAC Quantikine ELISA Kit
The recovery of I-TAC spiked to levels throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=4) | 100 | 91-111 |
EDTA Plasma (n=5) | 98 | 85-112 |
Heparin Plasma (n=5) | 98 | 90-106 |
Serum (n=5) | 106 | 93-117 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of I-TAC were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human CXCL11/I-TAC Quantikine ELISA Kit
Human CXCL11/I-TAC ELISA Cell Culture Supernate Standard Curve
Human CXCL11/I-TAC ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: CXCL11/I-TAC
Alternate Names
Gene Symbol
Additional CXCL11/I-TAC Products
Product Documents for Human CXCL11/I-TAC Quantikine ELISA Kit
Product Specific Notices for Human CXCL11/I-TAC Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.