Human IFN-gamma Quantikine HS ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # HSDIF0
Key Product Details
Assay Length
4.0 hours
Sample Type & Volume Required Per Well
Serum (100 µL), EDTA Plasma (0 Not Assigned), Heparin Plasma (100 µL)
Sensitivity
0.173 pg/mL
Assay Range
0.469-30 pg/mL (Serum, Plasma, EDTA Plasma, Heparin Plasma)
Product Summary for Human IFN-gamma Quantikine HS ELISA Kit
The Quantikine® HS Human IFN-gamma Immunoassay is a 4.0 hour solid-phase ELISA designed to measure human IFN-gamma in serum and plasma. It contains HEK293-expressed recombinant human IFN-gamma and antibodies raised against the recombinant protein. Results obtained using natural human IFN-gamma showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-gamma.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450 or 490nm (TMB or AP Substrate)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IFN-gamma.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians.
EDTA Plasma, Heparin Plasma, Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 1.83 | 6.10 | 12.8 | 2.15 | 6.33 | 12.4 |
| Standard Deviation | 0.084 | 0.187 | 0.253 | 0.210 | 0.421 | 0.898 |
| CV% | 4.6 | 3.1 | 2.0 | 9.8 | 6.7 | 7.2 |
Recovery for Human IFN-gamma Quantikine HS ELISA Kit
The recovery of human IFN-gamma spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 99 | 90-108 |
| Heparin Plasma (n=4) | 99 | 92-109 |
| Serum (n=4) | 97 | 85-109 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of human IFN-gamma were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human IFN-gamma Quantikine HS ELISA Kit
Human IFN-gamma HS Quantikine ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IFN-gamma
Long Name
Interferon gamma
Alternate Names
IFG, IFI, IFNG, IFNgamma
Entrez Gene IDs
Gene Symbol
IFNG
Additional IFN-gamma Products
Product Documents for Human IFN-gamma Quantikine HS ELISA Kit
Product Specific Notices for Human IFN-gamma Quantikine HS ELISA Kit
For research use only
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