Human IL-10 ELISA Kit - Quantikine Best Seller
R&D Systems, part of Bio-Techne | Catalog # D1000B
Key Product Details
Assay Length
3.5 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 µL), Serum (200 µL), EDTA Plasma (200 µL), Heparin Plasma (200 µL), Citrate Plasma (200 µL)
Sensitivity
3.9 pg/mL
Assay Range
7.8-500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Product Summary for Human IL-10 Quantikine ELISA Kit
The Quantikine Human IL-10 Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure IL-10 in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human IL-10 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-10 and does not cross-react with viral IL-10. Results obtained using natural human IL-10 showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate this kit can be used to determine relative mass values for natural human IL-10.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IL-10
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma - Forty serum and plasma samples from apparently healthy volunteers were
evaluated for the presence of human IL-10 in this assay. All samples measured less than the
lowest standard, 7.8 pg/mL. No medical histories were available for the donors used in this
study.
Cell Culture Supernates - Human peripheral blood mononuclear cells (1 x 106
cells/mL) were
cultured in RPMI supplemented with 10% fetal bovine serum, 50 μM beta-mercaptoethanol,
2 mM L-glutamine, 100 units/mL penicillin, 100 μg/mL streptomycin sulfate. The cells were
cultured unstimulated or stimulated with 10 μg/mL PHA for 1, 3, and 5 days. Aliquots of the cell
culture supernates were removed and assayed for human IL-10.
| Condition | Day 1 (pg/mL) | Day 3 (pg/mL) | Day 5 (pg/mL) |
| Unstimulated | 98 | 134 | 127 |
| Stimulated | 2756 | 3563 | 2255 |
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 24.4 | 79.6 | 223 | 23.6 | 74.2 | 227 |
| Standard Deviation | 1.6 | 3.9 | 5.5 | 1.8 | 4.9 | 12.7 |
| CV% | 6.6 | 4.9 | 2.5 | 7.6 | 6.6 | 5.6 |
Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 23.9 | 76.9 | 231 | 23.2 | 75.1 | 228 |
| Standard Deviation | 1.2 | 3.3 | 3.9 | 1.7 | 5.6 | 13.4 |
| CV% | 5.0 | 4.3 | 1.7 | 7.3 | 7.5 | 5.9 |
Recovery for Human IL-10 Quantikine ELISA Kit
The recovery of human IL-10 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 98 | 88-110 |
| Citrate Plasma (n=5) | 96 | 87-108 |
| EDTA Plasma (n=5) | 92 | 75-105 |
| Heparin Plasma (n=5) | 94 | 84-105 |
| Serum (n=5) | 100 | 92-110 |
Linearity
To assess the linearity of the assay, five samples were spiked with high concentrations of IL-10 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human IL-10 Quantikine ELISA Kit
Human IL-10 ELISA Cell Culture Supernate Standard Curve
Human IL-10 ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-10
IL-10 mediates its biological activities through a heteromeric receptor complex composed of
the type II cytokine receptor subunits IL-10 R alpha and IL-10 R beta. IL-10 R alpha is a 110 kDa
transmembrane glycoprotein that is expressed on lymphocytes, NK cells, macrophages,
monocytes, astrocytes, intestinal epithelial cells, cytotrophoblasts, and activated hepatic
stellate cells (8-13), while the 75 kDa transmembrane IL-10 R beta is widely expressed (14, 15). The
IL-10 dimer binds to two IL-10 R alpha chains, triggering recruitment of two IL-10 R beta chains (14, 15).
IL-10 R beta does not bind IL-10 directly but is required for signal transduction. IL-10 R beta also
associates with IL-20 R alpha, IL-22 R alpha1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26,
IL-28, and IL-29 (16-18).
The involvement of IL-10 in immunoregulation includes both suppressive and stimulatory
effects. It functions as an anti-inflammatory cytokine by inhibiting the expansion and
activation of Th1 cells and Th17 cells (19-21) and by promoting the development of M2
macrophages (21). Its expression by immunosuppressive regulatory T cells (Treg) and
regulatory B cells is important for Treg proliferation (19). Within a tumor microrenvironment,
however, IL-10 inhibits the expansion of Treg as well as myeloid-derived suppressor cells (22,
23). IL-10 induces the intratumoral accumulation and activation of CD8+ T cells (24, 25). IL-10
exerts protective effects including limiting tissue damage in arthritic inflammation (19) and
promoting muscle regeneration after injury (21), but it also contributes to the persistence of
viral infections (26). The levels of IL-10 are elevated in Sjogren's syndrome (saliva), primary CNS
lymphoma (cerebrospinal fluid), and ovarian cancer (serum and ascites) (27-29). Its levels are
decreased in the serum in patients with recurrent heart attacks or during preeclampsia and
also in the seminal fluid of infertile men (30-32).
Long Name
Interleukin 10
Alternate Names
CSIF, GVHDS, IL10, IL10A, TGIF
Entrez Gene IDs
Gene Symbol
IL10
Additional IL-10 Products
Product Documents for Human IL-10 Quantikine ELISA Kit
Product Specific Notices for Human IL-10 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.