Human IL-2 QuantiGlo ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # Q2000B
Key Product Details
Assay Length
5.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL)
Sensitivity
0.25 pg/mL
Assay Range
1.7-1250 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human IL-2 QuantiGlo ELISA Kit
The QuantiGlo Human IL-2 Chemiluminescent Immunoassay is a 5.5 hour solid phase ELISA designed to measure IL-2 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-2 and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human IL-2. Results obtained using natural human IL-2 showed linear curves that were parallel to the standard curves obtained using the recombinant QuantiGlo kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-2.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Chemiluminescent - Luminometer (Luminol)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IL-2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 5.8 | 175 | 884 | 5.9 | 165 | 795 |
| Standard Deviation | 0.19 | 5.6 | 23 | 0.46 | 11 | 59 |
| CV% | 3.3 | 3.2 | 2.6 | 7.8 | 6.7 | 7.4 |
Recovery for Human IL-2 QuantiGlo ELISA Kit
The recovery of IL-2 spiked to three different levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 102 | 99-105 |
| EDTA Plasma (n=4) | 97 | 95-100 |
| Heparin Plasma (n=4) | 97 | 94-104 |
| Serum (n=4) | 98 | 96-101 |
Linearity
To assess the linearity of the assay, four samples containing or spiked with high concentrations of IL-2 in various matrices were diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human IL-2 QuantiGlo ELISA Kit
Human IL-2 Chemiluminescent ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-2
The receptor for IL-2 (IL-2 R) is composed of three subunits, the 55 kDa CD25/IL-2 R alpha chain, the 70 kDa IL-2 R beta chain, and the 65 kDa Common gamma Chain (1, 3). IL-2 first binds to CD25, the binary complex then recruits IL-2 R beta and gammac to form the quaternary signaling complex (1, 14). In addition to IL-2, IL-2 R beta is used by IL-15 in its quaternary signaling complex. gammac also serves as a signaling receptor for IL-4, -7, -9, -15, and -21 (1, 3).
In vitro studies have shown an important role for IL-2 in T cell activation and expansion. In vivo, IL-2 is critical for the development, maintenance and function of regulatory T cells (Treg) which provide protection against autoimmune disease. On the other hand, IL-2 can also promote autoimmune inflammation in target organs through its roles in regulating the expression of T cell trafficking genes, and production of Th2 cytokines. Within the CD8+ T cell subset, IL-2 is essential for optimal primary responses and differentiation into terminal effector cells. IL-2 also promotes the development of activated CD8+ T cells into memory cells. (1).
Long Name
Interleukin 2
Alternate Names
Aldesleukin, IL2, Proleukin, TCGF
Entrez Gene IDs
Gene Symbol
IL2
Additional IL-2 Products
Product Documents for Human IL-2 QuantiGlo ELISA Kit
Product Specific Notices for Human IL-2 QuantiGlo ELISA Kit
For research use only
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