Human IL-5 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # D5000B
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Urine (100 µL)
Sensitivity
1.08 pg/mL
Assay Range
3.9-250 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Human IL-5 Quantikine ELISA Kit
The Quantikine Human IL-5 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-5 levels in cell culture supernates, serum, plasma, and urine. It contains Sf 21-expressed recombinant human IL-5 and antibodies raised against the recombinant factor and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-5 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-5.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IL-5
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 28.1 | 89.3 | 170 | 31.2 | 92.6 | 174 |
| Standard Deviation | 0.89 | 2.0 | 7.27 | 2.5 | 4.2 | 8.7 |
| CV% | 3.2 | 2.2 | 4.2 | 8.0 | 4.5 | 5.0 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 31.3 | 95.6 | 173 | 32.9 | 97.9 | 180 |
| Standard Deviation | 1.4 | 3.5 | 6.5 | 4.0 | 6.7 | 8.4 |
| CV% | 4.5 | 3.7 | 3.8 | 12.2 | 6.8 | 4.7 |
Recovery for Human IL-5 Quantikine ELISA Kit
The recovery of human IL-5 spiked to different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 101 | 95-107 |
| EDTA Plasma (n=4) | 104 | 86-118 |
| Heparin Plasma (n=4) | 101 | 87-112 |
| Serum (n=4) | 104 | 88-116 |
| Urine (n=4) | 96 | 91-106 |
Linearity
To assess the linearity of the assay, five samples were spiked with high concentrations of IL-5 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human IL-5 Quantikine ELISA Kit
Human IL-5 ELISA Cell Culture Supernate/Urine Standard Curve
Human IL-5 ELISA Serum/Plasma Standard Curve
Preparation and Storage
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-5
Long Name
Interleukin 5
Alternate Names
BCDF mu, BCGFII, EDF, Eo-CSF, IL5, TRF
Entrez Gene IDs
Gene Symbol
IL5
Additional IL-5 Products
Product Documents for Human IL-5 Quantikine ELISA Kit
Product Specific Notices for Human IL-5 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.