Human IL-6 QuantiGlo ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # Q6000B
Key Product Details
Assay Length
5.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Citrate Plasma (100 µL)
Sensitivity
0.35 pg/mL
Assay Range
0.48-1500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Product Summary for Human IL-6 QuantiGlo ELISA Kit
The QuantiGlo Human IL-6 Chemiluminescent Immunoassay is a solid phase ELISA that specifically measures human IL-6 in less than 5.5 hours. This kit is designed to measure IL-6 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-6 and antibodies raised against the recombinant factor. It has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-6 showed linear curves that were parallel to the standard curves obtained using the QuantiGlo kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-6. It has been observed in our laboratories that the measurement of human IL-6 is insensitive to the addition of the recombinant form of the human IL-6 soluble receptor.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Chemiluminescent - Luminometer (Luminol)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IL-6
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Four samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Four samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||||
|---|---|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 |
| n | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 1.21 | 10.7 | 118 | 766 | 1.56 | 13.2 | 142 | 752 |
| Standard Deviation | 0.07 | 0.32 | 4.15 | 37.5 | 0.15 | 1.02 | 8.93 | 53.7 |
| CV% | 5.8 | 3.0 | 3.5 | 4.9 | 9.6 | 7.7 | 6.3 | 7.1 |
Recovery for Human IL-6 QuantiGlo ELISA Kit
The recovery of IL-6 spiked to three different levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 103 | 98-113 |
| Citrate Plasma (n=4) | 87 | 82-96 |
| EDTA Plasma (n=4) | 89 | 82-95 |
| Heparin Plasma (n=4) | 88 | 80-97 |
| Serum (n=4) | 94 | 88-106 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of IL-6 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human IL-6 QuantiGlo ELISA Kit
Human IL-6 Chemiluminescent ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-6
IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a
ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to
IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization (39). gp130 is also a
component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (40). Soluble forms of
IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage (5). In a mechanism
known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-
expressing cells that lack cell surface IL-6 R alpha (5). Trans-signaling enables a wider range of
cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is
predominantly restricted to hepatocytes, monocytes, and resting lymphocytes (2, 5). Soluble
splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that
use gp130 as a co-receptor (5, 41).
IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response. IL-6 is almost solely
responsible for fever and the acute phase response in the liver, and it is important in the
transition from acute inflammation to either acquired immunity or chronic inflammatory
disease (1-5). When dysregulated, it contributes to chronic inflammation in conditions
such as obesity, insulin resistance, inflammatory bowel disease, arthritis, and sepsis (2, 5).
IL-6 modulates bone resorption and is a major effector of inflammatory joint destruction
in rheumatoid arthritis through its promotion of Th17 cell development and activity (1).
It contributes to atherosclerotic plaque development and destabilization as well as the
development of inflammation-associated carcinogenesis (1, 2). IL-6 can also function as an
anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise
(2). In addition, it enhances hematopoietic stem cell proliferation and the differentiation of
memory B cells and plasma cells (42).
Long Name
Interleukin 6
Alternate Names
BSF-2, BSF2, IFNB2, IL6, MGI-2A
Entrez Gene IDs
Gene Symbol
IL6
Additional IL-6 Products
Product Documents for Human IL-6 QuantiGlo ELISA Kit
Product Specific Notices for Human IL-6 QuantiGlo ELISA Kit
For research use only
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