Human MIF Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DMF00B
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), Platelet-poor EDTA Plasma (10 µL), Platelet-poor Heparin Plasma (10 µL), Urine (10 µL), Human Milk (10 µL)
Sensitivity
0.068 ng/mL
Assay Range
0.156-10 ng/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine, Human Milk)
Product Summary for Human MIF Quantikine ELISA Kit
The Quantikine Human MIF Immunoassay is a 4.5 hour solid phase ELISA designed to measure human MIF in cell culture supernates, serum, plasma, urine, and human milk. It contains E. coli-expressed recombinant human MIF and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human MIF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human MIF.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human MIF
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Human Milk, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 1.16 | 3.56 | 7.27 | 1.03 | 3.20 | 6.46 |
| Standard Deviation | 0.07 | 0.18 | 0.33 | 0.10 | 0.27 | 0.61 |
| CV% | 6.0 | 5.1 | 4.5 | 9.7 | 8.4 | 9.4 |
Recovery for Human MIF Quantikine ELISA Kit
The recovery of MIF spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 95 | 86-104 |
| Platelet-poor EDTA Plasma (n=5) | 95 | 80-108 |
| Platelet-poor Heparin Plasma (n=5) | 96 | 86-108 |
| Serum (n=4) | 99 | 94-104 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of MIF were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.\line
Scientific Data Images for Human MIF Quantikine ELISA Kit
Human MIF ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: MIF
Long Name
Macrophage Migration Inhibitory Factor
Alternate Names
EC 5.3.2.1, EC 5.3.3.12, GIFmacrophage migration inhibitory factor, GLIF, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, macrophage migration inhibitory factor (glycosylation-inhibiting factor), MMIF, Phenylpyruvate tautomerase
Gene Symbol
MIF
Additional MIF Products
Product Documents for Human MIF Quantikine ELISA Kit
Product Specific Notices for Human MIF Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.