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Mouse IgG2b ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00446

Novus Biologicals, part of Bio-Techne
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NBP3-00446

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

1.88 ng/mL

Assay Range

3.13 - 200 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

This kit recognizes Mouse IgG2b in samples. No significant cross-reactivity or interference between Mouse IgG2b and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 4.58%

Inter-Assay Precision (Precision between assays) 4.98%

Recovery for Mouse IgG2b ELISA Kit (Colorimetric)

Recovery

86 - 106%

Linearity

Mouse IgG2b ELISA Kit (Colorimetric)

Scientific Data Images for Mouse IgG2b ELISA Kit (Colorimetric)

Mouse IgG2b ELISA Kit (Colorimetric)

ELISA: Mouse IgG2b ELISA Kit (Colorimetric) [NBP3-00446] -

ELISA: Mouse IgG2b ELISA Kit (Colorimetric) [NBP3-00446] - Standard Curve Reference

Kit Contents for Mouse IgG2b ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G2B Fc

Additional IgG2B Products

Product Documents for Mouse IgG2b ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse IgG2b ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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