Human IL-12 p70/IL-12A ELISA Kit

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11729

Novus Biologicals, part of Bio-Techne
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NBP3-11729
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell culture supernatants (100 uL)

Sensitivity

1 pg/mL

Assay Range

3.16 - 1000 pg/mL

View all IL-12 p70 ELISA Kits »

Product Specifications

Kit Type

ELISA Kit

Reactivity

Human

Precision

Intra-Assay Precision (Precision within an assay) %CV < 6.7

Inter-Assay Precision (Precision between assays) %CV < 11.2

Recovery for Human IL-12 p70/IL-12A ELISA Kit

Recovery

94 - 107%

Scientific Data Images for Human IL-12 p70/IL-12A ELISA Kit

ELISA: Human IL-12 p70/IL-12A ELISA Kit [NBP3-11729]

ELISA: Human IL-12 p70/IL-12A ELISA Kit [NBP3-11729]

ELISA: IL-12 p70/IL-12A ELISA kit [NBP3-11729] - Representative standard curve.

Kit Contents for Human IL-12 p70/IL-12A ELISA Kit

  • Biotinylated detection mAb (MT704)
  • Buffers for washing and dilution and standard reconstitution
  • Pre-coated ELISA strip plates (mAbs MT86/221)
  • Recombinant human IL-12 p70 ELISA standard (lyophilized)
  • Streptavidin-HRP
  • Substrate (TMB)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-12 p70

Interleukin-12, or IL-12, also known as natural killer cell stimulatory factor (NKSF) and cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine that affects both natural killer (NK) cells and T lymphocytes. IL-12 is a heterodimer of subunit IL-12A p35 and subunit IL-12B p40 that is secreted by a wide variety of antigen presenting cells (APCs), including phagocytes, B cells and Langerhans cells. It is involved in mediating many aspects of antiviral immunity. In addition to stimulating T helper cell development, IL-12 is a potent inducer of interferon-gamma (IFN-gamma) production by T cells and NK cells. Increased IL-12 production is a consequence of EBV, CMV, LCMV or HSV infection. While it is unclear what separate effects can be attributed to each IL-12 subunit, evidence suggests IL-12A p35 is the biologically active component responsible for signal transduction via the IL-12 receptor.

Long Name

Interleukin 12 p70

Alternate Names

IL12 p70

Gene Symbol

IL12A

Additional IL-12 p70 Products

Product Documents for Human IL-12 p70/IL-12A ELISA Kit

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human IL-12 p70/IL-12A ELISA Kit

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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