Human MMP-15/MT2-MMP ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06940

Novus Biologicals, part of Bio-Techne
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NBP3-06940
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant (100 ul)

Sensitivity

0.09 ng/mL

Assay Range

0.16 - 10 ng/mL

View all MMP-15/MT2-MMP ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human MMP-15/MT2-MMP in samples. No significant cross-reactivity or interference between Human MMP-15/MT2-MMP and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 4.95

Inter-Assay Precision (Precision between assays) 4.47

Recovery for Human MMP-15/MT2-MMP ELISA Kit (Colorimetric)

Recovery

92-110 %

Linearity

Human MMP-15/MT2-MMP ELISA Kit (Colorimetric)

Scientific Data Images for Human MMP-15/MT2-MMP ELISA Kit (Colorimetric)

Human MMP-15/MT2-MMP ELISA Kit (Colorimetric)

ELISA: Human MMP-15/MT2-MMP ELISA Kit (Colorimetric) [NBP3-06940] -

ELISA: Human MMP-15/MT2-MMP ELISA Kit (Colorimetric) [NBP3-06940] - Standard Curve Reference

Kit Contents for Human MMP-15/MT2-MMP ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: MMP-15/MT2-MMP

MMPs are a group of enzymes involved in matrix degradation. Of the sixteen members of matrix metalloproteinase family ten exist in soluble form whereas MT MMPs exist as integral membrane proteins. MT1 MMp, MT2 MMP and MT3 MMP also known as MMP 14, MMP 15 and MMP 16 respectively. They contain a C-terminal transmembrane domain anchoring them to the cell surface. MT2 MMP plays a minor role in activation of pro-MMP 2 to its active form in breast carcinomas. Coexpression of MT1 MMP and MT2 MMP in advanced stages of breast carcinomas is indicative of possible involvement of MT2 MMP in its metastasis.

Long Name

Matrix Metalloproteinase 15/Membrane Type 2 MMP

Alternate Names

MMP15, MT2-MMP

Gene Symbol

MMP15

Additional MMP-15/MT2-MMP Products

Product Documents for Human MMP-15/MT2-MMP ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human MMP-15/MT2-MMP ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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