Mouse Endoglin/CD105 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MNDG00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (13 µL), EDTA Plasma (13 µL), Heparin Plasma (13 µL), Urine (25 µL)
Sensitivity
13.6 pg/mL
Assay Range
62.5-4000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Mouse Endoglin/CD105 Quantikine ELISA Kit
The Quantikine Mouse Endoglin Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse Endoglin in cell culture supernates, cell lysates, serum, plasma, and urine. It contains NS0-expressed recombinant mouse Endoglin and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural Endoglin showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Endoglin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse Endoglin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 32 | 32 | 32 |
| Mean (pg/mL) | 182 | 463 | 1472 | 176 | 552 | 1457 |
| Standard Deviation | 9.4 | 22.0 | 40.2 | 15.3 | 36.3 | 71.4 |
| CV% | 5.2 | 4.8 | 2.7 | 8.7 | 6.6 | 4.9 |
Recovery for Mouse Endoglin/CD105 Quantikine ELISA Kit
The recovery of mouse Endoglin spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Samples (n=7) | 102 | 93-114 |
| Cell Lysates (n=1) | 115 | - |
| EDTA Plasma (n=4) | 107 | 99-114 |
| Heparin Plasma (n=4) | 109 | 108-111 |
| Serum (n=4) | 107 | 100-117 |
| Urine (n=4) | 107 | 95-118 |
Linearity
To assess the linearity of the assay, four samples containing high concentrations of Endoglin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Mouse Endoglin/CD105 Quantikine ELISA Kit
Mouse Endoglin/CD105 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Endoglin/CD105
Alternate Names
CD105, ENG
Gene Symbol
ENG
Additional Endoglin/CD105 Products
Product Documents for Mouse Endoglin/CD105 Quantikine ELISA Kit
Product Specific Notices for Mouse Endoglin/CD105 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.