Mouse IGFBP-3 Quantikine ELISA Kit

Name: Mouse IGFBP-3 Quantikine ELISA Kit
Brand: R&D Systems
SKU: MGB300

R&D Systems, part of Bio-Techne | Catalog # MGB300

Submit a review

Product Datasheet / COA / SDS

100% Guarantee

Product Summary
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), EDTA Plasma (10 µL)

Sensitivity

16 pg/mL

Assay Range

78.1-5000 pg/mL (Cell Culture Supernates, EDTA Plasma)

View all IGFBP-3 ELISA Kits »

Product Summary for Mouse IGFBP-3 Quantikine ELISA Kit

The Quantikine Mouse IGFBP-3 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse IGFBP-3 in cell culture supernates and plasma. It contains NS0-expressed recombinant mouse IGFBP-3 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse IGFBP-3 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse IGFBP-3.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Reactivity

Mouse

Specificity

Natural and recombinant mouse IGFBP-3. This kit detects full-length and fragmented mouse IGFBP-3. It also detects IGF-I, IGF-II, IGF-I/ALS, and IGF-II/ALS complexed IGFBP-3.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean (pg/mL)	280	628	2653	283	602	2648
Standard Deviation	13.5	25.1	148	23.4	44.4	156
CV%	4.8	4.0	5.6	8.3	7.4	5.9

Recovery for Mouse IGFBP-3 Quantikine ELISA Kit

The recovery of mouse IGFBP-3 spiked to three levels throughout the range of the assay in the cell culture supernate samples was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Samples (n=4)	100	90-109

Linearity

To assess the linearity of the assay, samples containing high concentrations of mouse IGFBP-3 in each matrix were diluted with Calibrator Diluent and assayed.

Scientific Data Images for Mouse IGFBP-3 Quantikine ELISA Kit

Mouse IGFBP-3 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IGFBP-3

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. ALS (Acid Labile Subunit) is a liver-derived protein that exists in a ternary complex with Insulin-like Growth Factor (IGF)-binding Protein-3 (IGFBP-3) or IGFBP-5, and either IGF-I or IGF-II. ALS increases the half-life of IGF/IGFBP complexes in circulation.

Long Name

Insulin-like Growth Factor Binding Protein 3

Alternate Names

IGFBP3

Entrez Gene IDs

3486 (Human); 16009 (Mouse)

Gene Symbol