Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MLA00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL)
Sensitivity
2.5 pg/mL
Assay Range
4.69-300 pg/mL (Cell Culture Supernates, Serum)
Product Summary for Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit
The Quantikine Mouse IL-1 alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-1 alpha levels in cell culture supernates and serum. It contains E. coli-expressed recombinant mouse IL-1 alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-1 alpha accurately. Results obtained using natural mouse IL-1 alpha showed dose response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-1 alpha.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse IL-1 alpha
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 17 | 32 | 156 | 18 | 33 | 162 |
| Standard Deviation | 1.5 | 1.0 | 3.9 | 0.9 | 1.2 | 7.9 |
| CV% | 8.8 | 3.1 | 2.5 | 5.0 | 3.6 | 4.9 |
Recovery for Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit
The recovery of mouse IL-1 alpha spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=5) | 102 | 92-109 |
| Serum (n=6) | 94 | 88-103 |
Linearity
To assess the linearity of the assay, five or more samples spiked with various concentrations of mouse IL-1 alpha in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit
Mouse IL-1 alpha/IL-1F1 ELISA Standard Curve
Preparation and Storage
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-1 alpha/IL-1F1
Long Name
Interleukin 1 alpha
Alternate Names
BAF, IL-1A, IL-1F1, IL1, IL1 alpha, IL1A, IL1F1, LAF, LEM
Entrez Gene IDs
Gene Symbol
IL1A
Additional IL-1 alpha/IL-1F1 Products
Product Documents for Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit
Product Specific Notices for Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.