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Mouse IL-13 Culture Media Quantikine ELISA Best Seller

R&D Systems, part of Bio-Techne | Catalog # M1300CB

R&D Systems, part of Bio-Techne
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M1300CB-1
SM1300CB

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL)

Sensitivity

1.5 pg/mL

Assay Range

7.8-500 pg/mL (Cell Culture Supernates)

Product Summary for Mouse IL-13 Culture Media Quantikine ELISA

The Quantikine Mouse IL-13 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-13 levels in cell culture supernates. It contains recombinant mouse IL-13 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-13 accurately. Results obtained using natural mouse IL-13 showed dose curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-13.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Reactivity

Mouse

Specificity

Natural and recombinant mouse IL-13

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 33.9 100 432 31.6 93.4 405
Standard Deviation 1.1 2.7 11.2 2.5 7.2 17.7
CV% 3.2 2.7 2.6 7.9 7.7 4.4

Recovery for Mouse IL-13 Culture Media Quantikine ELISA

The recovery of mouse IL-13 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=13) 99 89-119

Linearity

To assess the linearity of the assay, samples containing and/or spiked with mouse IL-13 were diluted with Calibrator Diluent and assayed.

Mouse IL-13 ELISA Linearity

Scientific Data Images for Mouse IL-13 Culture Media Quantikine ELISA

Mouse IL-13 ELISA Standard Curve

Mouse IL-13 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-13

IL-13 (Interleukin-13) plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances by macrophages, fibroblasts, and endothelial cells. It promotes B cell activation, immunoglobulin class switching to IgE, and the upregulation of CD23/Fc epsilon RII. IL-13 signals through a receptor complex containing IL-13 R alpha 1 and IL-4 R alpha. This complex also functions as the type 2 IL-4 receptor. IL-13 R alpha 2 binds IL-13 and prevents IL-13 from signaling through the IL-13 R alpha 1/IL-4 R alpha complex. IL-13-bound IL-13 R alpha 2 can directly promote tumor cell invasiveness and the development of tissue fibrosis.

Long Name

Interleukin 13

Alternate Names

IL13, NC30, P600

Entrez Gene IDs

3596 (Human); 16163 (Mouse); 116553 (Rat); 574325 (Primate)

Gene Symbol

IL13

Additional IL-13 Products

Product Documents for Mouse IL-13 Culture Media Quantikine ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse IL-13 Culture Media Quantikine ELISA

For research use only

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.