Mouse TIM-1/KIM-1/HAVCR Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # MKM100
Key Product Details
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Urine (10 µL)
Sensitivity
2.18 pg/mL
Assay Range
7.81-500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Mouse TIM-1/KIM-1/HAVCR Quantikine ELISA Kit
The Quantikine Mouse TIM-1 Immunoassay is a 3.5 hour solid phase ELISA designed to measure mouse TIM-1 in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant mouse TIM-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse TIM-1 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse TIM-1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse TIM-1. The antibody components of this kit were raised against the short splice form of mouse TIM-1 (isoform 2) which has a 23 amino acid deletion following Pro182. This immunoassay detects both the short and long forms of mouse TIM-1.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma/Urine - Samples were evaluated for the presence of mouse TIM-1 in this assay
ND=Non-Detectable
| NSA Strain | Mean of Detectable (pg/mL) | % Detectable | Range (pg/mL) |
| Serum (n=20) | 17.2 | 90 | ND-42.5 |
| EDTA plasma (n=20) | 25.6 | 100 | 7.87-174 |
| Heparin plasma (n=20) | 18.0 | 95 | ND-42.8 |
| Urine (n=20) | 2248 | 100 | 456-8048 |
Serum samples from two Balb/c strain mice were evaluated in this assay and measured
27.5 pg/mL and 26.9 pg/mL. Additionally, serum samples from two C57 strain mice were
evaluated in this assay and measured 20.4 pg/mL and 14.4 pg/mL.
Urine samples from two Balb/c strain mice and one C57 strain mouse were evaluated in this
assay and measured 143,000 pg/mL, 159,000 pg/mL, and 726 pg/mL respectively.
Cell Culture Supernates - Kidneys from mice were removed, rinsed in 1X PBS, and kept on
ice in 1X PBS. Kidneys were then homogenized using a tissue homogenizer and seeded into
media containing RPMI 1640 supplemented with 10% fetal bovine serum, 2 mM L-glutamine,
100 U/mL penicillin, and 100 mg/mL streptomycin sulfate. Cells were cultured for the times
indicated. Aliquots of the cell culture supernates were removed and assayed for levels of
mouse TIM-1.
| Tissue Type | (pg/mL) |
| NSA mouse kidney (1 day) | 1006 |
| NSA mouse kidney (3 days) | 756 |
| Balb/c mouse kidney (2 days) | 1679 |
| C57 mouse kidney (2 days) | 24 |
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 24.2 | 61.8 | 207 | 23.2 | 60.0 | 190 |
| Standard Deviation | 0.8 | 1.6 | 6.8 | 2.5 | 4.2 | 13.2 |
| CV% | 3.3 | 2.6 | 3.3 | 10.8 | 7.0 | 6.9 |
Recovery for Mouse TIM-1/KIM-1/HAVCR Quantikine ELISA Kit
The recovery of TIM-1 spiked into various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 100 | 91-106 |
| EDTA Plasma (n=4) | 89 | 88-91 |
| Heparin Plasma (n=4) | 87 | 86-89 |
| Serum (n=4) | 95 | 93-97 |
| Urine (n=4) | 102 | 94-115 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse TIM-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Mouse TIM-1/KIM-1/HAVCR Quantikine ELISA Kit
Mouse KIM-1 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TIM-1/KIM-1/HAVCR
Long Name
T Cell Immunoglobulin Mucin-1
Alternate Names
CD365, HAVCR1, KIM-1, TIM1
Gene Symbol
HAVCR1
Additional TIM-1/KIM-1/HAVCR Products
Product Documents for Mouse TIM-1/KIM-1/HAVCR Quantikine ELISA Kit
Product Specific Notices for Mouse TIM-1/KIM-1/HAVCR Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.