Mouse Total MMP-3 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MMP300
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL)
Sensitivity
0.053 ng/mL
Assay Range
0.312-10 ng/mL (Cell Culture Supernates, Serum)
Product Summary for Mouse Total MMP-3 Quantikine ELISA Kit
The Quantikine Mouse MMP-3 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure total mouse MMP-3 (pro-, active, and TIMP-complexed MMP-3) in cell culture supernates and mouse serum. It contains NS0-expressed recombinant mouse MMP-3 and antibodies raised against the recombinant factor. Both antibodies also recognize recombinant mouse active MMP-3. Natural mouse MMP-3 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative levels of natural mouse MMP-3.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse MMP-3 (pro-, active, and TIMP-complexed)
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 0.76 | 3.17 | 4.72 | 0.82 | 3.29 | 4.99 |
| Standard Deviation | 0.04 | 0.05 | 0.09 | 0.08 | 0.19 | 0.37 |
| CV% | 5.3 | 1.6 | 1.9 | 9.8 | 5.8 | 7.4 |
Recovery for Mouse Total MMP-3 Quantikine ELISA Kit
The recovery of mouse MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 99 | 92-106 |
| Serum (n=4) | 98 | 93-107 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse MMP-3 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Mouse Total MMP-3 Quantikine ELISA Kit
Mouse Total MMP-3 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: MMP-3
The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).
Long Name
Matrix Metalloproteinase 3
Alternate Names
MMP3, Stromelysin 1
Gene Symbol
MMP3
Additional MMP-3 Products
Product Documents for Mouse Total MMP-3 Quantikine ELISA Kit
Product Specific Notices for Mouse Total MMP-3 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.