Human Pentraxin 2/SAP ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA1841

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell Culture Sample, CSF, Plasma, Serum, Urine (50 uL)

Sensitivity

40 pg/mL

Assay Range

0.078 to 5.0 ng/mL

View all Pentraxin 2/SAP ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

APCS (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human APCS.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.9%

Inter-Assay Precision (Precision between assays) CV% < 7.1%

Recovery for Human Pentraxin 2/SAP ELISA Kit (Colorimetric)

Recovery

95%

Scientific Data Images for Human Pentraxin 2/SAP ELISA Kit (Colorimetric)

ELISA: Pentraxin 2/SAP ELISA Kit [Biotin] [KA1841] - Standard curve

Kit Contents for Human Pentraxin 2/SAP ELISA Kit (Colorimetric)

  • Biotinylated Human Serum Amyloid P Antibody (50x): A 50-fold concentrated biotinylated polyclonal antibody against SAP.
  • Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substrate tetramethylbenzidine.
  • Human Serum Amyloid P Microplate: A 96-well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody against human SAP.
  • Human Serum Amyloid P Standard: Human SAP in a buffered protein base (lyophilized).
  • MIX Diluent Concentrate (10x): A 10-fold concentrated buffered protein base.
  • Sealing Tapes: Pressure-sensitive sealing tapes that can be cut to fit the format of the individual assay.
  • Stop Solution: A 0.5 N hydrochloric acid to stop the chromogen substrate reaction.
  • Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate.
  • Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Pentraxin 2/SAP

Serum Amyloid P (SAP) is a non-fibrillar plasma glycoprotein that belongs to the pentraxin family. It is universally found in amyloid deposits and this is probably due to its specific calcium-dependent binding to motifs present on all types of amyloid fibrils. SAP is also found to prevent fibrillar breakdown by enzymes and it is believed that it helps maintains stability of the amyloid deposits (1,2). It has been shown that SAP binds monocytes with high avidity, but does not bind to erythrocytes, NK cells, T lymphocytes or B lymphocytes (3). SAP production can be induced by exposure to IL-1, IL-6 and IFN-beta. The SAP-inducing activity was neutralized by antibodies to each of the recombinant cytokines (4).

Long Name

Serum Amyloid P Component

Alternate Names

APCS, PTX2, SAP

Gene Symbol

APCS

Additional Pentraxin 2/SAP Products

Product Documents for Human Pentraxin 2/SAP ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Pentraxin 2/SAP ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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