Human Perforin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00418

Novus Biologicals, part of Bio-Techne
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NBP3-00418
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.47 ng/mL

Assay Range

0.78 - 50 ng/mL

View all Perforin ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human Perforin in samples. No significant cross-reactivity or interference between Human Perforin and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 4.81%

Inter-Assay Precision (Precision between assays) 4.6%

Recovery for Human Perforin ELISA Kit (Colorimetric)

Recovery

87 - 107%

Linearity

Human Perforin ELISA Kit (Colorimetric)

Scientific Data Images for Human Perforin ELISA Kit (Colorimetric)

Human Perforin ELISA Kit (Colorimetric)

ELISA: Human Perforin ELISA Kit (Colorimetric) [NBP3-00418] -

ELISA: Human Perforin ELISA Kit (Colorimetric) [NBP3-00418] - Standard Curve Reference

Kit Contents for Human Perforin ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Perforin

Perforin (PRF1) is a 70 kD cytolytic protein that is the major protein constituent of cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Specifically, perforin enters the target cell's plasma membrand and creates a intercellular pore to mediate targeted cell lysis. PRF1 antibodies are useful tools for lysis research on both CTLs and NK cells.

Long Name

Perforin 1 (Pore Forming Protein)

Alternate Names

Cytolysin, FLH2, HPLH2, P1, PFP, PRF1

Gene Symbol

PRF1

Additional Perforin Products

Product Documents for Human Perforin ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Perforin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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