Mouse/Rat IL-6 ELISpot Kit

Name: Mouse/Rat IL-6 ELISpot Kit
Brand: R&D Systems
SKU: EL406

R&D Systems, part of Bio-Techne | Catalog # EL406

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Product Datasheet / COA / SDS

Key Product Details

Assay Type:	Quantitative Sandwich ELISA
Format:	96-well PVDF-backed microplate
Assay Length:	3 hours 15 mins to 4 hours 30 mins*
Sample Types:	Whole Cells
Specificity:	Please see the product datasheet

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Technical Data
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Product Summary for Mouse/Rat IL-6 ELISpot Kit

ELISpot kits are highly sensitive, microplate-based assays for the detection of cytokine secreting cells. This kit is designed for the detection and enumeration of mouse/rat IL-6. Complete ELISpot kits are ready-to-run and require no assay development or refinement.

This ELISpot assay employs a capture antibody specific for mouse/rat IL-6, pre-coated onto a PVDF-backed microplate. Appropriately stimulated cells are pipetted directly into the wells and the immobilized antibody in the immediate vicinity of the secreting cells binds secreted mouse/rat IL-6. Following wash steps and incubation with a biotinylated detection antibody, alkaline-phosphatase conjugated to streptavidin is added. Unbound enzyme is subsequently removed by washing and a substrate solution (BCIP/NBT) is added. A blue-black colored precipitate forms at the sites of cytokine localization and appears as spots, with each individual spot representing an individual mouse/rat IL-6 secreting cell. The spots can be counted with an automated ELISpot reader system or manually using a stereomicroscope.

Product Features for Mouse/Rat IL-6 ELISpot Kit

Detect and quantitate individual cells secreting mouse/rat IL-6
High sensitivity - ELISpot assays can measure responses with frequencies well below 1 in 100,000 cells
No in vitro expansion of cells required
High-throughput - ELISpot assays use only a small number of primary cells

Kit Contents for Mouse/Rat IL-6 ELISpot Kit

Mouse/rat IL-6 Microplate
Biotinylated Detection Antibody
Streptavidin conjugated to Alkaline Phosphatase
Dilution Buffers
Wash Buffer Concentrate
BCIP/NBT Chromogen
Mouse/rat IL-6 Positive Control

Other Reagents Required

Pipettes and pipette tips
Deionized or distilled water
Squirt bottle, manifold dispenser, or automated microplate washer
500 mL graduated cylinder
37 °C CO₂ incubator
Sterile culture media
Dissection microscope or an automated ELISpot reader

Formulation, Preparation and Storage

Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-6

IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 signals through a receptor complex of IL-6 R alpha and gp130. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha.

Item	Value
Long Name	Interleukin 6
Entrez Gene IDs	3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)
Alternate Names	B-cell differentiation factor, B-cell stimulatory factor 2, BSF-2, BSF2, CDF, CTL differentiation factor , HSF, IFN-beta-2, IFNB2, IL-6, IL6, Interferon beta-2, MGI-2A, hybridoma growth factor, interleukin 6 (interferon, beta 2), interleukin BSF-2, interleukin-6

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Product Documents for Mouse/Rat IL-6 ELISpot Kit

Product Datasheets

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COA

SDS

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⚠ WARNING: This product can expose you to chemicals including methanol, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov.