LDV FITC

Name: LDV FITC
Brand: Tocris Bioscience
SKU: 4577

Tocris Bioscience, part of Bio-Techne | Catalog # 4577

Citations (7)

COA / SDS

Fluorescent ligand for α4β1 (VLA-4)

Key Product Details

Description:	Fluorescent ligand for α4β1 (VLA-4)
Purity:	≥90% (HPLC)
Molecular Weight:	1368.54

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Technical Data
Citations

Biological Activity for LDV FITC

LDV FITC is a fluorescent ligand that binds to the α₄β₁ integrin (VLA-4) with high affinity (K_d values are 0.3 nM and 12 nM for binding to U937 cells in the presence and absence of Mn²⁺ respectively). Used to detect VLA-4 affinity and conformational changes. Wavelengths are compatible with use as an acceptor dye in TR-FRET assays, together with the CoraFluor™ 1 (Cat. No. 7920) TR-FRET donor.

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Technical Data for LDV FITC

M. Wt	1368.54
Formula	C₆₉H₈₁N₁₁O₁₇S
Single Letter Sequence	LDVPAAK (Modifications: Leu-1 N-2-[4-[[[(2-methylphenyl)amino]carbonyl]amino]phenyl]acetyl-, Lys-6 N-FITC conjugate)
Storage	Store at -20°C
Purity	≥90% (HPLC)
CAS Number	1207610-07-8
PubChem ID	91826076
InChi Key	PAAUNHNWVWSJKI-CBRXRBRLSA-N
Smiles	CC(C)C[C@H](NC(=O)Cc1ccc(NC(=O)Nc2ccccc2C)cc1)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCNC(=S)Nc1ccc(C(O)=O)c(c1)-c1c-2ccc(=O)cc-2oc2cc(O)ccc12)C(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for LDV FITC

Solubility

Soluble to 1mg/ml in PBS (pH 7.4)

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References for LDV FITC

References are publications that support the biological activity of the product.

Njus Conformational mAb as a tool for integrin ligand discovery. Assay Drug Dev.Technol. 2009 PMID: 19754304
Chigaev FRET detection of cellular α₄-integrin conformational activation. Biophys.J. 2003 PMID: 14645084
Chigaev Real time analysis of the affinity regulation of alpha 4-integrin. The physiologically activated receptor is intermediate in affinity between resting and Mn²⁺ or antibody activation. J.Biol.Chem. 2001 PMID: 11641394