Ocean Blue, SE

Name: Ocean Blue, SE
Brand: Tocris Bioscience
SKU: 6489

Tocris Bioscience, part of Bio-Techne | Catalog # 6489

COA / SDS

Blue fluorescent dye (Reactive group: NHS ester). Application: flow cytometry, fluorescence microscopy

Key Product Details

Description:	Blue fluorescent dye (Reactive group: NHS ester). Application: flow cytometry, fluorescence microscopy
Chemical Name:	3-Carboxy-6,8-difluoro-7-hydroxycoumaryl succinimidyl ester
Purity:	≥95% (HPLC)
Molecular Weight:	339.2

View Full Technical Details for Ocean Blue, SE

Technical Data
Citations

Biological Activity for Ocean Blue, SE

Key information: Ocean Blue, SE is a blue fluorescent dye supplied with an NHS ester reactive group for the labeling of primary amines.

Application: Flow cytometry, fluorescence microscopy.

Properties and Photophysical Data: Conjugates of this dye are strongly fluorescent even at neutral pH. Excitation and emission maxima (λ) are 405 nM and 455 nM, respectively; quantum yield = 0.78; extinction coefficient = 36,000 M^-1cm^-1; A280 correction factor = 0.15.

Please see the protocol for further information on protein/antibody labeling and conjugation.

Note: This product is typically prepared in DMSO.

Optical Data for Ocean Blue, SE

Emission Color	Blue
Brightness
λ_abs	405 nm
λ_em	455 nm
Extinction Coefficient (ε)	36000 M^-1cm^-1
Quantum Yield (φ)	0.78
Closest Laser line	405 nm
Reactive Group	NHS ester
Reactivity	Primary amines
Correction Factor 280	0.15
Application	Flow Cytometry, Fluorescence Microscopy

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for Ocean Blue, SE

M. Wt	339.2
Formula	C₁₄H₇F₂NO₇
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	215868-33-0
PubChem ID	56927770
InChi Key	NZYXABPVYJRICY-UHFFFAOYSA-N
Smiles	OC1=C(C=C2C=C(C(OC2=C1F)=O)C(ON3C(CCC3=O)=O)=O)F

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

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References for Ocean Blue, SE

References are publications that support the biological activity of the product.

Cox Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling. Biotechniques 2004 PMID: 14740493
Brandtzaeg The increasing power of immunohistochemistry and immunocytochemistry. J.Immunol.Methods 1998 PMID: 9760215
Haugland Antibody conjugates for cell biology. Curr.Protoc.Cell Biol. 2001 PMID: 18228336
Sun Synthesis of novel fluorinated coumarins: excellent UV-light excitable fluorescent dyes. Bioorg.Med.Chem.Lett. 1998 PMID: 9873685