Mouse IgG2a Isotype Control (M2A) [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-96889
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
ELISA, Flow Cytometry, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence
Label
Biotin
Antibody Source
Monoclonal Mouse IgG2A Clone # M2A
Concentration
LYOPH mg/ml
Product Summary for Mouse IgG2a Isotype Control (M2A) [Biotin]
Specificity
Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgM, and IgA. Biotin Conjugated Mouse IgG2a was proven by Dot Blot.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This antibody has been prepared from in vitro cell culture by selective precipitation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2a and anti-Mouse Serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgM, and IgA. Biotin Conjugated Mouse IgG2a was proven by Dot Blot.
This antibody has been prepared from in vitro cell culture by selective precipitation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2a and anti-Mouse Serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgM, and IgA. Biotin Conjugated Mouse IgG2a was proven by Dot Blot.
Scientific Data Images for Mouse IgG2a Isotype Control (M2A) [Biotin]
ELISA: Mouse IgG2a Isotype Control (M2A) [Biotin] [NBP1-96889] - ELISA Results of Mouse IgG2a isotype control (M2A) [Biotin]. Each well was coated in duplicate with 1.0 ug of Mouse IgG2a Isotype Control Biotin Conjugate. The working dilution is 364,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer, Streptavidin-HRP conjugated and TMB substrate.
Applications for Mouse IgG2a Isotype Control (M2A) [Biotin]
Application
Recommended Usage
Flow Cytometry
1:1000 - 1:5000
Fluorophore-linked immunosorbent assay
1:100 - 1:2000
Immunocytochemistry/ Immunofluorescence
1:1000 - 1:5000
Application Notes
This product has been tested in ELISA and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Selective Precipitation
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent).
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: IgG2A
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G2A Fc
Additional IgG2A Products
Product Documents for Mouse IgG2a Isotype Control (M2A) [Biotin]
Product Specific Notices for Mouse IgG2a Isotype Control (M2A) [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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