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Mouse IgG2B Isotype Control Best Seller

R&D Systems, part of Bio-Techne | Catalog # MAB004

R&D Systems, part of Bio-Techne
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MAB004

Key Product Details

Species Reactivity

Mouse

Applications

Control

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 20116

Product Summary for Mouse IgG2B Isotype Control

Immunogen

KLH

Specificity

Selected for use as mouse IgG2B isotype control. In direct ELISAs, does not cross-react with recombinant human (rh) 6Ckine, recombinant mouse (rm) 6Ckine, rhACT II (rhMIP-1 beta), rhBLC/BCA-1, rmBLC, rhBRAK, rmBRAK, rmC10, recombinant rat (rr) CINC-1, rrCINC-2 alpha, rrCINC-2 beta, rrCINC-3, rhCK beta8-1, rmCRG-2, rmCTACK, rhENA-78, rhEotaxin, rmEotaxin, rhEotaxin 2, rmEotaxin-2, rhEotaxin-3, rhFractalkine, rmFractalkine, rrFractalkine, rhGCP-2, rmGCP-2, rhGRO alpha, rhGRO beta, rhGRO gamma, rhHCC-1, rhHCC-4, rhI-309, rhIL-8, recombinant porcine IL-8, rhIP-10, rhI-TAC, rmI-TAC, rmJE, rmKC, rhLeukotactin-1, rrLIX, rhLymphotactin, rmLymphotactin, rmMARC, rhMCP-1, rhMCP-2, rhMCP-3, rhMCP-4, rmMCP-5, rhMDC, rmMDC, rhMIG, rmMIG, rhMIP-1 alpha, rmMIP-1 alpha, rmMIP-1 beta, rmMIP-1 gamma, rhMIP-1 delta, rmMIP-2, rhMIP-3 alpha, rmMIP-3 alpha, rrMIP-3 alpha, rhMIP-3 beta, rmMIP-3 beta, recombinant viral (rv) MIP-I, rvMIP-II, rvMIP-III, rhMPIF-1, rhNAP-2, rhParc, rhRANTES, rmRANTES, rhSDF-1 alpha, rmSDF-1 alpha, rhSDF-1 beta, rhTarc, rmTarc, rmTCA-3, rhTeck, rmTeck, rhVIC, or rmVIC.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Mouse IgG2B Isotype Control

Detection of Mouse IgG Isotype Control by Flow Cytometry

Detection of Mouse IgG Control by Flow Cytometry

K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human ITFG1 Monoclonal Antibody (Catalog # MAB89001, filled histogram) or Mouse IgG Isotype Control Antibody (Catalog # MAB004, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog F0101B).

Applications for Mouse IgG2B Isotype Control

Application
Recommended Usage

Control

Negative control for use in conjunction with R&D Systems antibodies. Use at the same concentration as the detection antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 8 reviews rated 4.6 using MAB004 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G2B Fc

Additional IgG2B Products

Product Documents for Mouse IgG2B Isotype Control

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse IgG2B Isotype Control

For research use only

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