Recombinant Canine CXCL8/IL-8 Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34908
Key Product Details
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 79 amino acids corresponding to CXCL8/IL-8 Source: E. coli
Uniprot ID: P41324
Amino Acid Sequence: AVLSRVSSEL RCQCIKTHST PFHPKYIKEL RVIDSGPHCE NSEIIVKLFN GNEVCLDPKE KWVQKVVQIF LKKAEKQDP
This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Level
Less than 1 EU/ug of CXCL8/IL-8 as determined by LAL method.
Predicted Molecular Mass
9.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
IL8 protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected murine BaF3 cells is in a concentration range of 0.15-0.75 ng/ml.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Canine CXCL8/IL-8 Protein
SDS-Page: Canine CXCL8/IL-8 Protein [NBP2-34908]
Formulation, Preparation and Storage
NBP2-34908
| Preparation Method | Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance. |
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.4. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: IL-8/CXCL8
Long Name
Interleukin 8
Alternate Names
CXCL8, GCP1, IL8, LAI, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP1, NCF, TCF, TSG1, IL-8/CXCL8
Gene Symbol
CXCL8
UniProt
Additional IL-8/CXCL8 Products
Product Documents for Recombinant Canine CXCL8/IL-8 Protein
Product Specific Notices for Recombinant Canine CXCL8/IL-8 Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...