Recombinant Porcine CXCL8/IL-8 Protein

Name: Recombinant Porcine CXCL8/IL-8 Protein
Brand: Novus Biologicals
SKU: NBP2-35234

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35234

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-8/CXCL8 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 78 amino acids corresponding to CXCL8/IL-8 Source: E. coli

Uniprot ID: P26894

Amino Acid Sequence: ARVSAELRCQ CINTHSTPFH PKFIKELRVI ESGPHCENSE IIVKLVNGKE VCLDPKEKWV QKVVQIFLKR TEKQQQQQ

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CXCL8/IL-8 as determined by LAL method.

Predicted Molecular Mass

9.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL8 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration range of 20-200 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Porcine CXCL8/IL-8 Protein

SDS-PAGE: Recombinant Porcine CXCL8/IL-8 Protein [NBP2-35234]

SDS-Page: Porcine CXCL8/IL-8 Protein [NBP2-35234]

Formulation, Preparation and Storage

NBP2-35234

Preparation Method	Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-8/CXCL8

Interleukin-8 (IL-8) is encoded by the IL8 gene and produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles. There are many receptors capable to bind IL-8, the most affinity to IL-8 are receptors CXCR1, and CXCR2. As a member of the CXC chemokine family, function of IL-8 is the induction of chemotaxis in its target cells, like neutrophil granulocytes, basophils, and T-cells. IL-8 is often associated with inflammation and has been cited as a proinflammatory mediator in gingivitis and psoriasis.

Long Name

Interleukin 8

Alternate Names

CXCL8, GCP1, IL8, LAI, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP1, NCF, TCF, TSG1, IL-8/CXCL8

Gene Symbol

CXCL8

Additional IL-8/CXCL8 Products

Product Documents for Recombinant Porcine CXCL8/IL-8 Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Porcine CXCL8/IL-8 Protein

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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