Recombinant Human/Rat/Bovine FGF-10, Animal-Free Protein

R&D Systems, part of Bio-Techne | Catalog # Qk003

Human/Rat/Bovine/Porcine
R&D Systems, part of Bio-Techne
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Qk003-0050
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Key Product Details

Source

E. coli

Accession #

O15520.1

Conjugate

Unconjugated

Applications

Bioactivity

View all FGF-10 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived FGF-10 protein

Purity

Single species with expected mass

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Predicted Molecular Mass

17 kDa

SDS-PAGE

Monomeric FGF-10 protein only

Activity

No significant difference between EC50 of reference and test lots

Mycoplasma

Negative when tested in both ribosomal RNA hybridization and luminescence assays

Scientific Data Images for Recombinant Human/Rat/Bovine FGF-10, Animal-Free Protein

Recombinant Human/Rat/Bovine FGF-10, Animal-Free Protein Bioactivity

FGF-10 activity is determined using the firefly luciferase reporter assay (*) in stably transfected HEK293T cells. Cells are treated in triplicate with a serial dilution of FGF-10. Firefly luciferase activity is measured and normalized. EC50 = 0.36 ng/ml (21.1 pM).*Promega pGL4.33[luc2P/SRE/Hygro] #E1340

Recombinant Human/Rat/Bovine FGF-10, Animal-Free Protein SDS-PAGE

FGF-10 migrates as a single band at 17 kDa in non-reducing (NR) conditions and upon reduction (R). No contaminating protein bands are visible.Purified recombinant human FGF-10 protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+ beta-mercaptoethanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250.

Formulation, Preparation and Storage

Qk003
Formulation Lyophilized from HEPES/NaCl/mannitol
Reconstitution Resuspend in water at >100 µg/ml, prepare single use aliquots, add carrier protein if desired.
Shipping The product is shipped lyophilized at ambient temperature, on ice blocks or on dryice. Shipping at ambient temperature does not affect the bioactivity or stability ofthe protein. Upon receipt, store immediately at the conditions stated below.
Stability & Storage Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles.

Background: FGF-10

The Fibroblast Growth Factors (FGFs) are heparin binding glycoproteins that exert a variety of biological activities toward cells of mesenchymal, neuronal, and epithelial origin. FGF-10 belongs to the subgroup of FGFs that also includes FGF-3, -7, and -22 (1). Mature human FGF-10 is an approximately 20 kDa protein that contains a serine-rich region near its N-terminus (2, 3). It shares 93% and 96% amino acid sequence identity with mouse and rat FGF-10, respectively. FGF-10 is secreted by mesenchymal cells and associates with extracellular FGF-BP (1, 4). It preferentially binds and activates epithelial cell FGF R2 (IIIb) and interacts more weakly with FGF R1 (IIIb) (5). The mitogenic and chemotactic properties of FGF-10 are critical in many tissues during embryogenesis. This includes limb bud initiation (6), palate development (7), branching morphogenesis and directional outgrowth of lung buds (8, 9), formation of the otic vesicle and chochlea (10), adipogenesis (11), and the development of prostate, mammary, lacrimal, and submandibular salivary glands (12 - 15). FGF R2 (IIIb) signaling in these responsive tissues is similarly important during embryogenesis (7, 10, 13 ‑ 15). The expression and function of FGF-10 are negatively regulated by Shh and BMP-4 in the developing lung (8, 9). Overlapping expression patterns and activities with FGF-3, -7,  and -8 suggest at least a partial redundancy in FGF‑10 biology (7, 10, 14, 15). FGF-10 induced signaling through FGF R2 (IIIb) also contributes to the progression of pancreatic cancer (16).

References

  1. Beenken, A. and M. Mohammadi (2009) Nat. Rev. Drug Discov. 8:235.
  2. Igarashi, M. et al. (1998) J. Biol. Chem. 273:13230.
  3. Emoto, H. et al. (1997) J. Biol. Chem. 272:23191.
  4. Beer, H.-D. et al. (2005) Oncogene 24:5269.
  5. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694.
  6. Min, H. et al. (1998) Genes Dev. 12:3156.
  7. Rice, R. et al. (2004) J. Clin. Invest. 113:1692.
  8. Bellusci, S. et al. (1997) Development 124:4867.
  9. Weaver, M. et al. (2000) Development 127:2695.
  10. Pirvola, U. et al. (2000) J. Neurosci. 20:6125.
  11. Sakaue, H. et al. (2002) Genes Dev. 16:908.
  12. Donjacour, A.A. et al. (2003) Dev. Biol. 261:39.
  13. Mailleux, A.A. et al. (2002) Development 129:53.
  14. Makarenkova, H.P. et al. (2000) Development 127:2563.
  15. Jaskoll, T. et al. (2005) BMC Dev. Biol. 5:11.
  16. Nomura, S. et al. (2008) Br. J. Cancer 99:305.

Long Name

Fibroblast Growth Factor 10

Alternate Names

FGF10, KGF-2, KGF2

Entrez Gene IDs

2255 (Human); 14165 (Mouse); 25443 (Rat)

Gene Symbol

FGF10

UniProt

O15520.1

Additional FGF-10 Products

Product Documents for Recombinant Human/Rat/Bovine FGF-10, Animal-Free Protein

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human/Rat/Bovine FGF-10, Animal-Free Protein

The above product was manufactured, tested and released by R&D System's contract manufacturer, Qkine Ltd, at 1 Murdoch House, Cambridge, UK, CB5 8HW. The product is for research use only and not for the diagnostic or theraputic use.

For research use only

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