Recombinant Mouse BAFF/BLyS/TNFSF13B Protein Best Seller

R&D Systems, part of Bio-Techne | Catalog # 8876-BF

Analyzed by SEC-MALS
R&D Systems, part of Bio-Techne
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8876-BF-010/CF
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8876-BF-010
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Key Product Details

Source

NS0

Accession #

Q9WU72

Structure / Form

Noncovalently-linked homotrimer

Conjugate

Unconjugated

Applications

Bioactivity

View all BAFF/BLyS/TNFSF13B Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse BAFF/BLyS/TNFSF13B protein
Ala127-Leu309

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala127

Predicted Molecular Mass

21 kDa

SDS-PAGE

19-23 kDa (monomer) & 38-47 kDa (non-reducible dimer), reducing conditions

Activity

Measured in a cell proliferation assay using anti-IgM stimulated mouse B cells.
The ED50 for this effect is 0.1‑0.5 ng/mL in the presence of goat anti‑mouse IgM µ chain.

Scientific Data Images for Recombinant Mouse BAFF/BLyS/TNFSF13B Protein

Recombinant Mouse BAFF/BLyS/TNFSF13B Protein SEC-MALS.

Recombinant Mouse BAFF/BLyS/TNFSF13B (Catalog # 8876-BF) has a molecular weight (MW) of 62.6 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Recombinant Mouse BAFF/BLyS/TNFSF13B Protein Bioactivity

Recombinant Mouse BAFF/BLyS/TNFSF13B Protein Bioactivity

Recombinant Mouse BAFF/BLyS/TNFSF13B (Catalog # 8876-BF) induces mouse B cell proliferation. The ED50 for this effect is 0.1‑0.5 ng/mL.The ED50 for the two competitors is >5 ng/mL, which is more than 25-fold weaker.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 8876-BF
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and TCEP with BSA as a carrier protein..
Reconstitution Reconstitute at 100-500 μg/mL in sterile water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 8876-BF/CF
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and TCEP.
Reconstitution Reconstitute at 100-500 μg/mL in sterile water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BAFF/BLyS/TNFSF13B

B-cell activating factor (BAFF), also known as BLyS, TALL-1, THANK, and TNFSF13B, is a 32 kDa transmembrane glycoprotein in the TNF ligand superfamily. It is involved in multiple aspects of immune system regulation, particularly towards B cells (1, 2). Mature mouse BAFF consists of a 47 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 241 aa extracellular domain (ECD) with a stalk region and one TNF-like domain (3, 4). Within aa 127-309 of the ECD, mouse BAFF shares 72% aa sequence identity with human BAFF. It can be expressed as a homotrimer or as a heteromer in association with the related TNFSF member APRIL (4, 5). A 18 kDa fragment containing the TNF-like domain can be released by proteolysis between Arg126 and Ala127 (4). Soluble BAFF is stored intracellularly in neutrophils and released upon inflammatory stimulation (6). Alternative splicing generates an isoform termed deltaBAFF that lacks 19 aa between the proteolytic cleavage site and the TNF-like domain. deltaBAFF can form heteromers with BAFF and negatively regulates BAFF function (7). BAFF is produced by many hematopoietic cell types including by monocytes, macrophages, neutrophils, dendritic cells, and T cells and also by adipocytes (1, 2, 8). Both BAFF and APRIL are functional ligands for the TNF receptor superfamily members BCMA and TACI, and BAFF additionally binds and signals through BAFF R (9, 10). All three receptors are primarily expressed by B cells (10). BAFF plays a critical role in the development and survival of B lineage cells (2, 11, 12). Mice that over-express BAFF exhibit elevated B cell numbers, increased formation and size of germinal centers, and symptoms of autoimmunity (13). Soluble BAFF is elevated in B cell malignancies, autoimmunity, and other immune disorders (1). In addition, BAFF costimulates T cell activation, promotes a Th1 biased immune response, and promotes the expansion of Treg cells (14-16). BAFF also promotes monocyte survival, proinflammatory cytokine secretion, and differentiation to macrophages (17).

References

  1. Lied, G.A. and A. Berstad (2011) Scand. J. Immunol. 73:1.
  2. Mackay, F. et al. (2010) Immunol. Rev. 237:205.
  3. Moore, P.A. et al. (1999) Science 285:260.
  4. Schneider, P. et al. (1999) J. Exp. Med. 189:1747.
  5. Roschke, V. et al. (2002) J. Immunol. 169:4314.
  6. Scapini, P. et al. (2003) J. Exp. Med. 197:297.
  7. Gavin, A.L. et al. (2003) J. Biol. Chem. 278:38220.
  8. Alexaki, V.-I. et al. (2009) J. Immunol. 183:5948.
  9. Yu, G. et al. (2000) Nat. Immunol. 1:252.
  10. Thompson, J.S. et al. (2001) Science 293:2108.
  11. Schiemann, B. et al. (2001) Science 293:2111.
  12. Litinskiy, M.B. et al. (2002) Nat. Immunol. 3:822.
  13. Batten, M. et al. (2000) J. Exp. Med. 192:1453.
  14. Huard, B. et al. (2001) J. Immunol. 167:6225.
  15. Sutherland, A.P.R. et al. (2005) J. Immunol. 174:5537.
  16. Walters, S. et al. (2009) J. Immunol. 182:793.
  17. Chang, S.K. et al. (2006) Blood 108:2687.

Long Name

B cell Activating Factor

Alternate Names

BLyS, CD257, TALL1, THANK, TNFSF13B, ZTNF4

Entrez Gene IDs

10673 (Human); 24099 (Mouse); 498666 (Rat); 102123214 (Cynomolgus Monkey)

Gene Symbol

TNFSF13B

UniProt

Q9WU72

Additional BAFF/BLyS/TNFSF13B Products

Product Documents for Recombinant Mouse BAFF/BLyS/TNFSF13B Protein

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Mouse BAFF/BLyS/TNFSF13B Protein

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