Recombinant Rat TNF RI/TNFRSF1A Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7990-TR

R&D Systems, part of Bio-Techne
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7990-TR-025
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Key Product Details

Source

NS0

Accession #

P22934

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all TNF RI/TNFRSF1A Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived rat TNF RI/TNFRSF1A protein
Rat TNF RI/TNFRSF1A
(Leu30-Ala211)
Accession # P22934		 IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu30

Predicted Molecular Mass

47.3 kDa (monomer)

SDS-PAGE

60-65 kDa, reducing conditions

Activity

Measured by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221.
The ED50 for this effect is 0.5-3 ng/mL in the presence of 0.1 ng/mL of Recombinant Rat TNF‑ alpha (Catalog # 510-RT).

Formulation, Preparation and Storage

7990-TR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNF RI/TNFRSF1A

TNF receptor 1 (TNF RI; also called TNF R‑p55/p60 and TNFRSF1A) is a 55 kDa type I transmembrane protein member of the TNF receptor superfamily, designated TNFRSF1A (1, 2). Rat TNF RI is a 461 amino acid (aa) protein that contains a 21 aa signal sequence, a 190 aa extracellular domain (ECD) with a PLAD (pre‑ligand assembly domain) that mediates constitutive dimer/trimer formation, followed by four CRD (cysteine-rich domains), a 23 aa transmembrane domain, and a 227 aa cytoplasmic sequence that contains a neutral sphingomyelinase activation domain and a death domain (3, 4). The ECD of rat TNF RI shares 69%, 88%, 66%, 68% and 75% aa sequence identity with human, mouse, canine, feline and porcine TNF RI, respectively. Both TNF RI and TNF RII (TNFRSF1B) are widely expressed and contain four TNF‑ alpha trimer-binding CRD in their ECD. However, TNF RI is thought to mediate most of the cellular effects of TNF‑ alpha (3). It is essential for proper development of lymph node germinal centers and Peyer’s patches, and for combating intracellular pathogens such as Listeria (1, 2, 5). TNF RI is also a receptor for TNF‑ beta/TNFSF1B (lymphotoxin‑ alpha) (6). TNF RI is stored in the Golgi and translocates to the cell surface following pro‑inflammatory stimuli (7). TNF‑ alpha stabilizes TNF RI and induces its sequestering in lipid rafts, where it activates NF kappaB and is cleaved by ADAM-17/TACE (8  9, 16). Release of the 28‑34 kDa TNF RI ECD also occurs constitutively and in response to products of pathogens such as LPS, CpG DNA or S. aureus protein A (1, 10‑12). Full‑length TNF RI may also be released in exosome‑like vesicles (13). Release helps to resolve inflammatory reactions, since it down‑regulates cell surface TNF RI and provides soluble TNF RI to bind TNF‑ alpha (10, 14, 15). Exclusion from lipid rafts causes endocytosis of TNF RI complexes and induces apoptosis (1). Mutations of human TNF R1 can result in inflammatory episodes known as TRAPS (TNFR‑associated periodic syndrome) (7).

References

  1. Pfeffer, K. (2003) Cytokine Growth Factor Rev. 14:185.
  2. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1.
  3. Chan, F.K. et al. (2000) Science 288:2351.
  4. Lewis, M. et al. (1991) Proc. Natl. Acad. Sci. USA 88:2830.
  5. Peschon, J.J. et al. (1998) J. Immunol. 160:943.
  6. Banner, D.W et al. (1993) Cell 73: 431.
  7. Turner, M.D. et al. (2012) Biosci. Rep. 32:105.
  8. Legler, D.F. et al. (2003) Immunity 18:655.
  9. Tellier, E. et al. (2006) Exp. Cell Res. 312:3969.
  10. Xanthoulea, S. et al. (2004) J. Exp. Med. 200:367.
  11. Jin, L. et al. (2000) J. Immunol. 165:5153.
  12. Gomez, M.I. et al. (2006) J. Biol. Chem. 281:20190.
  13. Islam, A. et al. (2006) J. Biol. Chem. 281:6860.
  14. Garton, K.J. et al. (2006) J. Leukoc. Biol. 79:1105.
  15. McDermott, M.F. et al. (1999) Cell 97:133.

Long Name

Tumor Necrosis Factor Receptor I

Alternate Names

CD120a, TNFRI, TNFRSF1A

Entrez Gene IDs

7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)

Gene Symbol

TNFRSF1A

UniProt

P22934

Additional TNF RI/TNFRSF1A Products

Product Documents for Recombinant Rat TNF RI/TNFRSF1A Fc Chimera Protein, CF

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