Rat IgG NorthernLights™ NL493-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # NL015
NorthernLights 493 Fluorochrome-labeled Antibody, X Absorbed
Conjugate
Catalog #
Key Product Details
Species Reactivity
Rat
Applications
Flow Cytometry, Immunocytochemistry, Immunohistochemistry
Label
NL493 (Excitation = 493 nm, Emission = 514 nm)
Antibody Source
Polyclonal Goat IgG
Product Summary for Rat IgG NorthernLights™ NL493-conjugated Antibody
Immunogen
Rat IgG heavy and light chains
Specificity
Rat IgG (H+L)
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Rat IgG NorthernLights™ NL493-conjugated Antibody
Detection of Rat IgG Primary Antibody by Immunocytochemistry
Detection of human Vimentin by incubating cells with 8 μg/mL of Rat anti-Human Vimentin monoclonal antibody (MAB2105) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Rat IgG NorthernLights™ NL493-conjugated secondary antibody for 1 hour at room temperature. Note strong positive staining in A172 cells (left panel, positive control) and a lack of non-specific background staining in the absence of primary antibodies (right panel, negative control).Applications for Rat IgG NorthernLights™ NL493-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
Use at a 1:200 dilution
Immunocytochemistry
Use at a 1:200 dilution
Immunohistochemistry
Use at a 1:200 dilution
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
*Contains <0.1% Sodium Azide, which is not hazardous at
this concentration according to GHS classifications. Refer to the Safety Data
Sheet
(SDS) for additional information and handling instructions.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: IgG
Long Name
Immunoglobulin G
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Additional IgG Products
Product Documents for Rat IgG NorthernLights™ NL493-conjugated Antibody
Product Specific Notices for Rat IgG NorthernLights™ NL493-conjugated Antibody
For research use only
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