Simple Plex Human GFAP Cartridge

Name: Simple Plex Human GFAP Cartridge
Brand: ProteinSimple
SKU: SPCKB-HF-000970

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-HF-000970

Product Datasheet / COA

Simple Plex Human GFAP assay kit for use on Ella instrument. Contains cartridge, sample diluent SD06, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cerebrospinal Fluid (25 uL)
Sensitivity:	14.4 pg/mL
Assay Range:	72.1 - 110,000 pg/mL (Cerebrospinal Fluid)
Simple Plex Human GFAP Cartridge Summary:	Simple Plex assay for the detection of human GFAP in cerebrospinal fluid (CSF)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human GFAP Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human GFAP Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human GFAP.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	40	40
Mean (pg/mL)	603	27073	709	30982
Standard Deviation	18.7	1217	78.1	3591
CV%	3.1	4.5	11	11.6

Linearity

Samples containing and/or spiked with high concentrations of human GFAP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human GFAP Cartridge

	Human GFAP Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GFAP

Glial Fibrillary Acidic Protein (GFAP) is a type III intermediate filament protein. GFAP is the predominant component of astrocyte intermediate filaments in the central nervous system and is often used as an astrocytic marker. It has also been detected in the glial cells of the enteric nervous system and some Schwann cells in the peripheral nervous system.

Item	Value
Long Name	Glial Fibrillary Acidic Protein
Entrez Gene IDs	2670 (Human); 14580 (Mouse); 24387 (Rat)
Alternate Names	ALXDRD, FLJ45472, GFAP, GFAP astrocytes, glial fibrillary acidic protein

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.