Simple Plex Human Perforin Cartridge

Name: Simple Plex Human Perforin Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-006982

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-006982

Simple Plex Human Perforin assay kit contains cartridge, sample diluent SD13, and wash buffer. Part # starting with ST01 ships in 2-3 days. Sample type indicated with PS (Plasma/Serum).

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (5 uL), EDTA Plasma (5 uL), Heparin Plasma (5 uL)
Sensitivity:	0.75 pg/mL
Assay Range:	3.14 - 12,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human Perforin Cartridge Summary:	Simple Plex assay for the detection of human Perforin in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human Perforin Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human Perforin Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human Perforin.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested sixteen times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	23	25
Mean (pg/mL)	92.9	3901	90.7	4145
Standard Deviation	2.07	74.9	7.41	371
CV%	2.2	1.9	8.2	8.9

Linearity

Samples containing and/or spiked with high concentrations of human Perforin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Human Perforin Cartridge

	Human Perforin Simple Plex Assay Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Perforin

Item	Value
Long Name	Perforin 1 (Pore Forming Protein)
Entrez Gene IDs	5551 (Human); 18646 (Mouse); 50669 (Rat)
Alternate Names	Cytolysin, FLH2, HPLH2, HPLH2lymphocyte pore forming protein, Lymphocyte pore-forming protein, MGC65093, P1, P1PFN1, PFP, PFPcytolysin, PRF1, Perforin, perforin 1 (pore forming protein), perforin-1

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.