Simple Plex Human TNF RI/TNFRSF1A Cartridge

Name: Simple Plex Human TNF RI/TNFRSF1A Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000434

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000434

Simple Plex Human TNF RI/TNFRSF1A assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Cerebrospinal Fluid (25 uL)
Sensitivity:	0.1 pg/mL
Assay Range:	0.89 - 3,390 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)
Simple Plex Human TNF RI/TNFRSF1A Cartridge Summary:	Simple Plex assay for the detection of human TNF RI in cell culture supernatan (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human TNF RI/TNFRSF1A Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human TNF RI/TNFRSF1A Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human TNF RI.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	24	22
Mean (pg/mL)	20.1	963	19.5	971
Standard Deviation	0.748	63.5	2.3	97.5
CV%	3.7	6.6	11.8	10

Linearity

Samples containing and/or spiked with high concentrations of human TNF RI were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human TNF RI/TNFRSF1A Cartridge

	Human TNF RI/TNFRSF1A Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TNF RI/TNFRSF1A

TNF RI (TNF receptor 1; TNF R-p55/p60) is a transmembrane receptor for TNF-alpha and Lymphotoxin-alpha/TNF-beta. A soluble decoy form of the receptor is shed by ADAM-17/TACE in response to inflammatory stimulation. TNF RI signaling through NFkB is essential for the development of lymph node germinal centers and Peyer’s patches and for combating intracellular pathogens such as Listeria.

Item	Value
Long Name	Tumor Necrosis Factor Receptor I
Entrez Gene IDs	7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)
Alternate Names	CD120a, CD120a antigen, FPF, TNF RI, TNF-R, TNF-R-I, TNF-R1, TNF-R55, TNF-RI, TNFARMGC19588, TNFR-I, TNFR1TBP1, TNFR55, TNFR60, TNFRI, TNFRSF1A, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor type I, p55, p55-R, p60, tumor necrosis factor binding protein 1, tumor necrosis factor receptor 1A isoform beta, tumor necrosis factor receptor superfamily member 1A, tumor necrosis factor receptor superfamily, member 1A, tumor necrosis factor receptor type 1, tumor necrosis factor-alpha receptor

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.