Simple Plex Mouse IL-5 Cartridge

Name: Simple Plex Mouse IL-5 Cartridge
Brand: ProteinSimple
SKU: SPCKB-MP-002846

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-002846

Product Datasheet / COA

Simple Plex Mouse IL-5 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Mouse Cell Culture Supernates (10 uL), Mouse Serum (25 uL), Mouse EDTA Plasma (25 uL), Mouse Heparin Plasma (25 uL)
Sensitivity:	0.17 pg/mL
Assay Range:	0.45 - 4,290 pg/mL (Mouse Cell Culture Supernates, Mouse Serum, Mouse EDTA Plasma, Mouse Heparin Plasma)
Simple Plex Mouse IL-5 Cartridge Summary:	Simple Plex assay for the detection of mouse IL-5 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Mouse IL-5 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Mouse IL-5 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Mouse
Specificity	Natural and recombinant mouse IL-5.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Mouse Cell Culture Supernates, Mouse Serum, Mouse EDTA Plasma, Mouse Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	47	46
Mean (pg/mL)	28.3	1593	24.8	1537
Standard Deviation	1.65	107	2.23	127
CV%	5.8	6.7	9	8.2

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Mouse EDTA Plasma (n=5)	109	98-120
Mouse Heparin Plasma (n=5)	107	98-111
Mouse Serum (n=3)	106	101-115

Linearity

Samples containing and/or spiked with high concentrations of mouse IL-5 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Mouse IL-5 Cartridge

	Mouse IL-5 Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-5

IL-5 (Interleukin-5) is a secreted cytokine that is expressed as a covalent dimer. It is produced by activated Th2 cells, eosinophils, mast cells, and iNKT cells and promotes eosinophilic and basophilic differentiation and activation. IL-5 signals through a receptor complex containing IL-5 R alpha and the Common beta Chain/CD131 on eosinophils and basophils. Naturally occurring soluble forms of IL-5 R alpha retain the ability to bind IL-5 and limit its bioavailability.

Item	Value
Long Name	Interleukin 5
Entrez Gene IDs	3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)
Alternate Names	B-cell differentiation factor I, BCDF mu, BCGFII, EDF, Eo-CSF, Eosinophil differentiation factor, IL-5, IL-5T-cell replacing factor, IL5, TRF, TRFB cell differentiation factor I, interleukin 5 (colony-stimulating factor, eosinophil), interleukin-5

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.