Simple Plex Mouse/Rat TREM-2 Cartridge

Name: Simple Plex Mouse/Rat TREM-2 Cartridge
Brand: ProteinSimple
SKU: SPCKB-MP-008555

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-008555

Simple Plex Mouse/Rat TREM-2 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.

Customize Your Simple Plex Cartridge

Create your custom Simple Plex cartridge for up to 8 targets. Choose from more than 250 targets across multiple species.

Build My Cartridge

Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Cerebrospinal Fluid (10 uL)
Sensitivity:	0.036 pg/mL
Assay Range:	5.24 - 8,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)
Simple Plex Mouse/Rat TREM-2 Cartridge Summary:	Simple Plex assay for the detection of mouse and rat Triggering Receptor Expressed on Myeloid cells 2 (TREM-2) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Mouse/Rat TREM-2 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Mouse/Rat TREM-2 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Mouse, Rat
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	74	74
Mean (pg/mL)	64.8	2887	60.2	2812
Standard Deviation	4.9	189	5.42	283
CV%	7.5	6.5	9	10.1

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	102	97-107

Linearity

Samples containing and/or spiked with high concentrations of mouse/rat TREM-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Mouse/Rat TREM-2 Cartridge

	Mouse/Rat TREM-2 Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TREM2

The TREM family of receptors regulates the activity of various cell types of the immune system including neutrophils, monocyte/macrophages, microglia, and dendritic cells. The receptors are members of the immunoglobulin superfamily, characterized structurally by a single V-set immunoglobulin (Ig) domain and a short cytoplasmic tail. TREMs associate with the DAP12 signal adapter, an immunoreceptor tyrosine-based activation motif- (ITAM)-containing protein that is phosphorylated and transduces signals when activated.

Item	Value
Long Name	Triggering Receptor Expressed on Myeloid Cells 2
Entrez Gene IDs	54209 (Human); 102133279 (Cynomolgus Monkey)
Alternate Names	PLOSL2, TREM-2, TREM-2triggering receptor expressed on myeloid cells 2a, TREM2, Trem2a, Trem2b, Trem2c, Triggering receptor expressed on monocytes 2, triggering receptor expressed on myeloid cells 2

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.