2-Phospho-L-ascorbic acid trisodium salt

Name: 2-Phospho- L -ascorbic acid trisodium salt
Brand: Tocris Bioscience
SKU: 5778

Tocris Bioscience, part of Bio-Techne | Catalog # 5778

Citations (1)

COA / SDS

Ascorbic acid derivative; maintains differentiation potential in bone marrow-derived MSCs

Key Product Details

Description:	Ascorbic acid derivative; maintains differentiation potential in bone marrow-derived MSCs
Chemical Name:	2-(Dihydrogen phosphate)-L-ascorbic acid sodium salt
Purity:	≥95% (HPLC)
Molecular Weight:	322.05

View Full Technical Details for 2-Phospho-L-ascorbic acid trisodium salt

Technical Data
Citations

Biological Activity

2-Phospho-L-ascorbic acid trisodium salt is a stable ascorbic acid derivative used in cell culture; in combination with FGF-2, maintains differentiation potential in bone marrow-derived mesenchymal stem cells (MSC) through increased expression of HGF. Also exhibits synergistic protection of hMSCs under oxidative stress in combination with N-acetylcysteine (Cat. No. 5619).

Technical Data

M. Wt	322.05
Formula	C₆H₆Na₃O₉P
Storage	Desiccate at RT
Purity	≥95% (HPLC)
CAS Number	66170-10-3
PubChem ID	10990876
InChi Key	VWCFDYSMDMCPLU-OFBPEYICSA-K
Smiles	[O-]C([C@]([C@@H](O)CO)([H])O1)=C(OP([O-])([O-])=O)C1=O.[Na+].[Na+].[Na+]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water	32.2	100

Preparing Stock Solutions

The following data is based on the product molecular weight 322.05.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.11 mL	15.53 mL	31.05 mL
5 mM	0.62 mL	3.11 mL	6.21 mL
10 mM	0.31 mL	1.55 mL	3.11 mL
50 mM	0.06 mL	0.31 mL	0.62 mL

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Reconstitution Calculator

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References

References are publications that support the biological activity of the product.

Bae L-ascorbic acid 2-phosphate and fibroblast growth factor-2 treatment maintains differentiation potential in bone marrow-derived mesenchymal stem cells through expression of hepatocyte growth factor. Growth Factors 2015 PMID: 25714612
Li Synergistic protection of N-acetylcysteine and ascorbic acid 2-phosphate on human mesenchymal stem cells against mitoptosis, necroptosis and apoptosis. Sci.Rep. 2015 PMID: 25909282
Falcon An in vitro cord formation assay identifies unique vascular phenotypes associated with angiogenic growth factors. PLoS One 2014 PMID: 25210890