A23187, free acid

Name: A23187, free acid
Brand: Tocris Bioscience
SKU: 1234
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 1234

(1)

Citations (10)

COA / SDS

Calcium ionophore

Key Product Details

Description:	Calcium ionophore
Alternative Names:	Calcimycin
Chemical Name:	5-(Methylamino)-2-[[2R,3R,6S,8S,9R,11R)-3,9,11-trimethyl-8-[(1S)-1-methyl-2-oxo-2-(1H-pyrrol-2-yl)-ethyl]-1,7-dioxaspiro[5.5]undec-2-yl]methyl]-4-benzoxazolecarboxylic acid
Purity:	≥98% (HPLC)
Molecular Weight:	523.63

View Full Technical Details for A23187, free acid

Technical Data
Citations / Reviews (1)

Biological Activity for A23187, free acid

A23187, free acid is a calcium ionophore that induces Ca²⁺-dependent cell death by increasing intracellular calcium concentration. Promotes intracelllular ROS generation and platelet particle formation (fragmentation) in vitro and in vivo. Can be used to induce autophagy in mammalian cells.

Technical Data for A23187, free acid

M. Wt	523.63
Formula	C₂₉H₃₇N₃O₆
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	52665-69-7
PubChem ID	11957499
InChi Key	HIYAVKIYRIFSCZ-CYEMHPAKSA-N
Smiles	[H][C@@]1([C@H](C)C(C5=CC=CN5)=O)[C@H](C)C[C@@H](C)[C@@]2(O[C@H](CC3=NC(C(C(O)=O)=C(NC)C=C4)=C4O3)[C@H](C)CC2)O1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for A23187, free acid

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	2.62	5

Preparing Stock Solutions for A23187, free acid

The following data is based on the product molecular weight 523.63.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.05 mM	38.19 mL	190.97 mL	381.95 mL
0.25 mM	7.64 mL	38.19 mL	76.39 mL
0.5 mM	3.82 mL	19.10 mL	38.19 mL
2.5 mM	0.76 mL	3.82 mL	7.64 mL

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References for A23187, free acid

References are publications that support the biological activity of the product.

Ding Differential effects of endoplasmic reticulum stress-induced autophagy on cell survival. J.Biol.Chem. 2007 PMID: 17135238
Nardi Platelet particle formation by anti-GPIIIa49-66 Ab, Ca2+ ionophore A23187, and phorbol myristate acetate is induced by reactive oxygen species and inhibited by dexameth. blockade of platelet phospholipase A2, 12-lipoxygenase, and Blood 2007 PMID: 17545506
Sakanashi Zn²⁺, derived from cell preparation, partly attenuates Ca²⁺-dependent cell death induced by A23187, calcium ionophore, in rat thymocytes. Toxicol.In Vitro 2009 PMID: 19124067
Kajitani Mechanism of A23187-induced apoptosis in HL-60 cells: dependency on mitochondrial permeability transition but not NADPH oxidase. Biosci.Biotechnol.Bichem. 2007 PMID: 7535265