Bafilomycin A1

Name: Bafilomycin A1
Brand: Tocris Bioscience
SKU: 1334
Rating: 4 (3 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 1334

(3)

Citations (235)

COA / SDS

H+-ATPase (vacuolar) inhibitor

Key Product Details

Description:	H⁺-ATPase (vacuolar) inhibitor
Chemical Name:	(3Z,5E,7R,8S,9S,11E,13E,15S,16R)-8-Hydroxy-16-[(1S,2R,3S)-2-hydroxy-1-methyl-3-[(2R,4R,5S,6R)-tetrahydro-2,4-dihydroxy-5-methyl-6-(1-methylethyl)-2H-pyran-2-yl]butyl]-3,15-dimethoxy-5,7,9,11-tetramethyloxacyclohexadeca-3,5,11,13-tetraen-2-one
Purity:	≥95% (HPLC)
Molecular Weight:	622.84

View Full Technical Details for Bafilomycin A1

Technical Data
Citations / Reviews (3)

Biological Activity for Bafilomycin A1

Bafilomycin A1 is a highly potent, selective inhibitor of vacuolar H⁺-ATPases (IC₅₀ = 0.6 - 1.5 nM in bovine chromaffin granules). Selective for v-ATPase over other ATP hydrolyzing enzymes such as F-ATPases and the H⁺/K⁺-ATPase (P-ATPase). Thought to inhibit autophagy either by blocking autophagosome-lysosome fusion (in H4IIE cells), or by blocking lysosomal degradation. Bafilomycin A1 accelerates wound healing in db/db mice. Low dose Bafilomycin A1 attenuates patient-derived CD34+CD19+ leukemia stem cells (LSC) and inhibits LSC proliferation in an animal model. Bafilomycin A1 impairs Zika virus infection in vitro.

Technical Data for Bafilomycin A1

M. Wt	622.84
Formula	C₃₅H₅₈O₉
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	88899-55-2
PubChem ID	72311
InChi Key	WEEFNMFMNMASJY-UHFFFAOYSA-M
Smiles	O[C@H]([C@H](C)/C=C(C)/C=C1OC)[C@@H](C)C/C(C)=C/C=C\[C@H](OC)C([C@@H](C)[C@H]([C@@H]([C@@]2(O)C[C@@H](O)[C@H](C)[C@@H]([C@H](C)C)O2)C)O)OC/1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Bafilomycin A1

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	4.98

Preparing Stock Solutions for Bafilomycin A1

The following data is based on the product molecular weight 622.84.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.08 mM	20.07 mL	100.35 mL	200.69 mL
0.4 mM	4.01 mL	20.07 mL	40.14 mL
0.8 mM	2.01 mL	10.03 mL	20.07 mL
4 mM	0.40 mL	2.01 mL	4.01 mL

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References for Bafilomycin A1

References are publications that support the biological activity of the product.

Xu Bafilomycin A1 targets patient-derived CD34 + CD19 + leukemia stem cells. Haematologica 2020 PMID: 31097633
Sabino Bafilomycin A1 and U18666A efficiently impair ZIKV infection in vitro. Viruses 2019 PMID: 31174294
Wang Bafilomycin A1 accelerates chronic refractory wound healing in db/db mice. Biomed.Res.Int. 2020 PMID: 32714982
Mauthe Chloroquine inhibits autophagic flux by decreasing autophagosome-lysosome fusion. Autophagy 2018 PMID: 29940786
Fass Microtubules support production of starvation-induced autophagosomes but not their targeting and fusion with lysosomes. J.Biol.Chem. 2006 PMID: 16963441
Yamamoto Bafilomycin A1 prevents maturation of autophagic vacuoles by inhibiting fusion between autophagosomes and lysosomes in rat hepatoma cell line, H-4-II-E cells. Cell Struct.Funct. 1998 PMID: 9639028
Gagliardi Chemistry and stucture activity relationships of bafilomycin A₁, a potent and selective inhibitor of the vacuolar H⁺-ATPase. Curr.Med.Chem. 1999 PMID: 10519916