BromoTag^® AGB3

BromoTag^® ABG3 is a selective "Bump & Hole" TAG Degrader (DC₅₀ 210 - 290 nM, protein degradation t½ = 113 min, D_max = 92%). BromoTag AGB3 comprises a VHL E3 ligase ligand joined by a linker to a (+)-JQ1 (Cat. No. 4499) derivative (the "bump" bearer) that selectively binds the Brd4^{BD2 L387A} variant (the "hole"). In cell lines expressing a fusion of the target protein with the tag, Brd4^{BD2 L387A}, BromoTag AGB3 drives formation of a ternary complex between VHL and the "BromoTagged" protein, leading to ubiquitination of the protein and its subsequent proteasomal degradation.

BromoTag^® is a registered trademark of the University of Dundee and is used under license.

Brd4^{BD2 L387A} can be expressed as a fusion with a target protein of interest using genome engineering techniques via CRISPR-mediated locus-specific knock-in. See protocol for more information.

Sold under exclusive licence from the University of Dundee