C25-140

Name: C25-140
Brand: Tocris Bioscience
SKU: 7715

Tocris Bioscience, part of Bio-Techne | Catalog # 7715

COA / SDS

TRAF6-Ubc13 interaction inhibitor

Key Product Details

Description:	TRAF6-Ubc13 interaction inhibitor
Chemical Name:	3-[3,5-Dimethyl-1-(3-methyl-1,2,4-triazolo[4,3-b]pyridazin-6-yl)-1H-pyrazol-4-yl]-1-[4-(phenylmethyl)-1-piperidinyl]-1-propanone
Purity:	≥98% (HPLC)
Molecular Weight:	457.57

View Full Technical Details for C25-140

Technical Data
Citations

Biological Activity for C25-140

C25-140 binds directly to TNF receptor-associated factor 6 (TRAF6) and inhibits its interaction with ubiquitin-conjugating enzyme Ubc13. C25-140 reduces TRAF6-Ubc13 activity and decreases NF-κB activation and inflammatory signaling in vitro. C25-140 exhibits selectivity for TRAF6 over other E3 ligases except cIAP1. In in vivo mouse models of autoimmune psoriasis and rheumatoid arthritis, the compound improves disease symptoms. The compound also suppresses the pro-inflammatory signaling activity of IL-17A and prevents its inhibitory effect on the anti-HBV action of IFN-α HepG2 cells.

Technical Data for C25-140

M. Wt	457.57
Formula	C₂₆H₃₁N₇O
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1358099-18-9
PubChem ID	53093165
InChi Key	HFKVRXVHZGBRKF-UHFFFAOYSA-N
Smiles	O=C(N1CCC(CC=2C=CC=CC2)CC1)CCC=3C(=NN(C4=NN5C(=NN=C5C)C=C4)C3C)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for C25-140

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	22.88	50
	ethanol	4.58	10

Preparing Stock Solutions for C25-140

The following data is based on the product molecular weight 457.57.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	4.37 mL	21.85 mL	43.71 mL
2.5 mM	0.87 mL	4.37 mL	8.74 mL
5 mM	0.44 mL	2.19 mL	4.37 mL
25 mM	0.09 mL	0.44 mL	0.87 mL

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References for C25-140

References are publications that support the biological activity of the product.

Zhang Interleukin-17A pretreatment attenuates the anti-hepatitis B virus efficacy of interferon-alpha by reducing activation of the interferon-stimulated gene factor 3 transcriptional complex in hepatitis B virus-expressing HepG2 cells. Virol.J. 2022 PMID: 35144643
Brenke Targeting TRAF6 E3 ligase activity with a small-molecule inhibitor combats autoimmunity. J.Biol.Chem. 2018 PMID: 29950522