Dorsomorphin dihydrochloride

Name: Dorsomorphin dihydrochloride
Brand: Tocris Bioscience
SKU: 3093
Rating: 5 (3 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 3093

(3)

Citations (217)

COA / SDS

Potent AMPK inhibitor; also BMP type I receptor inhibitor

Key Product Details

Description:	Potent AMPK inhibitor; also BMP type I receptor inhibitor
Alternative Names:	Compound C,BML-275
Chemical Name:	6-[4-[2-(1-Piperidinyl)ethoxy]phenyl]-3-(4-pyridinyl)-pyrazolo[1,5-a]pyrimidine dihydrochloride
Purity:	≥98% (HPLC)
Molecular Weight:	472.41

View Full Technical Details for Dorsomorphin dihydrochloride

Technical Data
Citations / Reviews (3)

Biological Activity for Dorsomorphin dihydrochloride

Dorsomorphin dihydrochloride is a potent inhibitor of AMP-activated protein kinase (AMPK) (K_i = 109 nM). Displays no significant activity on several structurally related kinases including ZAPK, SYK, PKCθ, PKA and JAK3. Inhibits AMPK activation induced by AICAR (Cat. No. 2840) and Metformin (Cat. No. 2864). Dorsomorphin dihydrochloride also inhibits bone morphogenetic protein (BMP) type I receptors (ALK2, ALK3 and ALK6), promotes cardiomyogenesis in mouse embryonic stem cells (ESCs) in vitro and promotes neural differentiation of hPSCs as part of a chemical cocktail. The compound can also be used in protocols for the chemical reprogramming of somatic cells to iPSCs.Shown to induce autophagy in cancer cell lines via a mechanism independent of AMPK inhibition. Also used in a protocol to generate brain organoids from human iPSCs.

Compound Libraries for Dorsomorphin dihydrochloride

Dorsomorphin dihydrochloride is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Kinase Inhibitor Library, Tocriscreen Epigenetics Library and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for Dorsomorphin dihydrochloride

M. Wt	472.41
Formula	C₂₄H₂₅N₅O.2HCl
Storage	Desiccate at RT
Purity	≥98% (HPLC)
CAS Number	1219168-18-9
PubChem ID	49761481
InChi Key	RJDVIJJQKMGPMV-UHFFFAOYSA-N
Smiles	C(C=N2)(C4=CC=C(OCCN5CCCCC5)C=C4)=CN1C2=C(C3=CC=NC=C3)C=N1.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Dorsomorphin dihydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	47.24	100
	DMSO	9.45	20 with gentle warming

Preparing Stock Solutions for Dorsomorphin dihydrochloride

The following data is based on the product molecular weight 472.41.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.12 mL	10.58 mL	21.17 mL
5 mM	0.42 mL	2.12 mL	4.23 mL
10 mM	0.21 mL	1.06 mL	2.12 mL
50 mM	0.04 mL	0.21 mL	0.42 mL

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References for Dorsomorphin dihydrochloride

References are publications that support the biological activity of the product.

Schafer An in vivo neuroimmune organoid model to study human microglia phenotypes. Cell 2023 PMID: 37172564
Chen Chemically defined neural conversion of human pluripotent stem cells. Methods Mol.Biol. 2019 PMID: 30656621
Guan Chemical reprogramming of human somatic cells to pluripotent stem cells. Nature 2022 PMID: 35418683
Diekmann A reliable and efficient protocol for human pluripotent stem cell differentiation into the definitive endoderm based on dispersed single cells. Stem Cells Dev. 2015 PMID: 25137387
Hao Dorsomorphin, a selective small molecule inhibitor of BMP signaling, promotes cardiomyogenesis in embryonic stem cells. PLoS ONE 2008 PMID: 18682835
Vucicevic Compound C induces protective autophagy in cancer cells through AMPK inhibition-independent blockade of Akt/mTOR pathway. Autophagy 2011 PMID: 20980833
Yu Dorsomorphin inhibits BMP signals required for embryogenesis and iron metabolism. Nat.Chem.Biol. 2008 PMID: 18026094
Zhou Role of AMP-activated protein kinase in mechanism of MetF. action. J.Clin.Invest. 2001 PMID: 11602624