NI 57

Name: NI 57
Brand: Tocris Bioscience
SKU: 5546

Tocris Bioscience, part of Bio-Techne | Catalog # 5546

COA / SDS

Potent and selective BRPF bromodomain inhibitor

Key Product Details

Description:	Potent and selective BRPF bromodomain inhibitor
Chemical Name:	4-Cyano-N-(1,3-dimethyl-2-oxo-1,2-dihydroquinolin-6-yl)-2-methoxybenzene-1-sulfonamide
Purity:	≥98% (HPLC)
Molecular Weight:	383.42

View Full Technical Details for NI 57

Technical Data
Citations

Biological Activity for NI 57

NI 57 is a potent and selective BRPF bromodomain inhibitor (K_d values are 31, 108 and 408 nM, for BRPF1B, 2 and 3, respectively, which exhibits >32-fold selectivity for BRPFs over BRD9 and other non-Class IV bromodomains. NI 57 inhibits RANKL-induced differentiation of primary murine bone marrow cells and human primary monocytes into osteoclasts.

Licensing Information

This probe is supplied in conjunction with the Structural Genomics Consortium. For further characterization details, please visit the NI 57 probe summary on the SGC website.

Compound Libraries for NI 57

NI 57 is also offered as part of the Tocriscreen Epigenetics Library. Find out more about compound libraries available from Tocris.

External Portal Information

Chemicalprobes.org is a portal that offers independent guidance on the selection and/or application of small molecules for research. The use of NI 57 is reviewed on the chemical probes website.

Technical Data for NI 57

M. Wt	383.42
Formula	C₁₉H₁₇N₃O₄S
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1883548-89-7
PubChem ID	91827373
InChi Key	UEMQPCYDWCSVCU-UHFFFAOYSA-N
Smiles	O=S(NC1=CC=C(N(C)C(C(C)=C2)=O)C2=C1)(C3=C(OC)C=C(C#N)C=C3)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for NI 57

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	38.34	100

Preparing Stock Solutions for NI 57

The following data is based on the product molecular weight 383.42.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.61 mL	13.04 mL	26.08 mL
5 mM	0.52 mL	2.61 mL	5.22 mL
10 mM	0.26 mL	1.30 mL	2.61 mL
50 mM	0.05 mL	0.26 mL	0.52 mL

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Reconstitution Calculator

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References for NI 57

References are publications that support the biological activity of the product.

Meier Selective targeting of bromodomains of the bromodomain-PHD fingers family impairs osteoclast differentiation. ACS Chem.Biol. 2017 PMID: 28849908
Igoe Design of a chemical probe for the bromodomain and plant homeodomain finger-containing (BRPF) family of proteins. J.Med.Chem. 2017 PMID: 28714688