OF 1

Name: OF 1
Brand: Tocris Bioscience
SKU: 5289

Tocris Bioscience, part of Bio-Techne | Catalog # 5289

COA / SDS

Selective BRPF1B and BRPF2 inhibitor

Key Product Details

Description:	Selective BRPF1B and BRPF2 inhibitor
Chemical Name:	4-Bromo-N-(2,3-dihydro-6-methoxy-1,3-dimethyl-2-oxo-1H-benzimidazol-5-yl)-2-methylbenzenesulfonamide
Purity:	≥98% (HPLC)
Molecular Weight:	440.31

View Full Technical Details for OF 1

Technical Data
Citations

Biological Activity for OF 1

OF 1 is a selective BRPF1B and BRPF2 bromodomain inhibitor (K_d values are 100 and 500 nM respectively). Exhibits 39-fold selectivity for BRPF1B and BRPF2 over BRD4. Accelerates FRAP recovery at 5 μM in a BRPF2 FRAP assay. Inhibits RANKL-induced differentiation of primary murine bone marrow cells and human primary monocytes into osteoclasts.

Licensing Information

This probe is supplied in conjunction with the Structural Genomics Consortium. For further characterization details, please visit the OF 1 probe summary on the SGC website.

Compound Libraries for OF 1

OF 1 is also offered as part of the Tocriscreen Epigenetics Library. Find out more about compound libraries available from Tocris.

Technical Data for OF 1

M. Wt	440.31
Formula	C₁₇H₁₈BrN₃O₄S
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	919973-83-4
PubChem ID	35397514
InChi Key	YUNQZQREIHWDQT-UHFFFAOYSA-N
Smiles	CN1C2=C(C=C(OC)C(NS(C3=C(C)C=C(Br)C=C3)(=O)=O)=C2)N(C)C1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for OF 1

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	8.81	20

Preparing Stock Solutions for OF 1

The following data is based on the product molecular weight 440.31.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	11.36 mL	56.78 mL	113.56 mL
1 mM	2.27 mL	11.36 mL	22.71 mL
2 mM	1.14 mL	5.68 mL	11.36 mL
10 mM	0.23 mL	1.14 mL	2.27 mL

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Reconstitution Calculator

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References for OF 1

References are publications that support the biological activity of the product.

Meier Selective targeting of bromodomains of the bromodomain-PHD fingers family impairs osteoclast differentiation. ACS Chem.Biol. 2017 PMID: 28849908