RITA

Name: RITA
Brand: Tocris Bioscience
SKU: 2443

Tocris Bioscience, part of Bio-Techne | Catalog # 2443

Citations (4)

COA / SDS

MDM2-p53 interaction inhibitor

Key Product Details

Description:	MDM2-p53 interaction inhibitor
Alternative Names:	NSC 652287
Chemical Name:	5,5'-(2,5-Furandiyl)bis-2-thiophenemethanol
Purity:	≥97% (HPLC)
Molecular Weight:	292.37

View Full Technical Details for RITA

Technical Data
Citations

Biological Activity for RITA

RITA is an anti-tumor agent that binds wild-type p53 (K_d = 1.5 nM) preventing p53-MDM2 (HDM2) interaction. Induces p53 accumulation and stimulates apoptosis in tumor cell lines expressing wild-type p53 in vitro and in vivo. Inhibits HPV-E6-mediated proteasomal degradation. Suppresses expression of cervical carcinoma HeLa xenografts in vivo.

Technical Data for RITA

M. Wt	292.37
Formula	C₁₄H₁₂O₃S₂
Storage	Store at -20°C
Purity	≥97% (HPLC)
CAS Number	213261-59-7
PubChem ID	374536
InChi Key	KZENBFUSKMWCJF-UHFFFAOYSA-N
Smiles	OCC1=CC=C(S1)C1=CC=C(O1)C1=CC=C(CO)S1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for RITA

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	29.23	100
	ethanol	7.31	25

Preparing Stock Solutions for RITA

The following data is based on the product molecular weight 292.37.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.42 mL	17.10 mL	34.20 mL
5 mM	0.68 mL	3.42 mL	6.84 mL
10 mM	0.34 mL	1.71 mL	3.42 mL
50 mM	0.07 mL	0.34 mL	0.68 mL

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References for RITA

References are publications that support the biological activity of the product.

Espinoza-Fonseca Targeting MDM2 by the small molecule RITA: towards the development of new multi-target drugs against cancer. Theor.Biol.Med.Mod.
Zhao Rescue of p53 function by small-molecule RITA in cervical carcinoma by blocking E6-mediated degradation. Cancer Res. 2010 PMID: 20395210
Issaeva Small molecule RITA binds to p53, blocks p53-HDM-2 interaction and activates p53 function in tumours. Nature Med.
Rivera Selective toxicity of the tricyclic thiophene NSC 652287 in renal carcinoma cell lines: differential accumulation and metabolism. Biochem.Pharmacol. 1999 PMID: 10230772