SPOP-i-6lc

Name: SPOP-i-6lc
Brand: Tocris Bioscience
SKU: 7498

Tocris Bioscience, part of Bio-Techne | Catalog # 7498

COA / SDS

SPOP E3 ubiquitin ligase inhibitor

Key Product Details

Description:	SPOP E3 ubiquitin ligase inhibitor
Chemical Name:	1,5-Dihydro-2-imino-10-methyl-N-[2-(4-methyl-1-piperazinyl)ethyl]-5-oxo-1-[2-(2-thienyl)ethyl]-2H-dipyrido[1,2-a:2',3'-d]pyrimidine-3-carboxamide
Purity:	≥98% (HPLC)
Molecular Weight:	505.64

View Full Technical Details for SPOP-i-6lc

Technical Data
Citations

Biological Activity for SPOP-i-6lc

SPOP-i-6lc is a selective SPOP E3 ubiquitin ligase inhibitor (IC₅₀ = 2.1 μM and 3.5 μM, in A498 and OS-RC-2 cell lines, respectively). In vitro, SPOP-i-6lc suppresses viability and proliferation of A498 and OS-RC-2 kidney cancer cell lines. SPOP-i-6lc leads to the accumulation of tumor suppressors PTEN and DUSP7 and decreased levels of phosphorylated AKT and ERK in clear-cell renal cell carcinoma (ccRCC) cell lines.

Technical Data for SPOP-i-6lc

M. Wt	505.64
Formula	C₂₆H₃₁N₇O₂S
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	2136270-56-7
PubChem ID	155532456
InChi Key	CQWPQOGWIPGPOA-UHFFFAOYSA-N
Smiles	CN1CCN(CC1)CCNC(C2=CC3=C(N(C2=N)CCC4=CC=CS4)N=C5N(C3=O)C=CC=C5C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for SPOP-i-6lc

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	ethanol	2.53	5
	DMSO	5.06	10

Preparing Stock Solutions for SPOP-i-6lc

The following data is based on the product molecular weight 505.64.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	19.78 mL	98.88 mL	197.77 mL
0.5 mM	3.96 mL	19.78 mL	39.55 mL
1 mM	1.98 mL	9.89 mL	19.78 mL
5 mM	0.40 mL	1.98 mL	3.96 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for SPOP-i-6lc

References are publications that support the biological activity of the product.

Dong Structure-activity relationship of SPOP inhibitors against kidney cancer. J.Med.Chem. 2020 PMID: 32297747