Wnt-C59

Name: Wnt-C59
Brand: Tocris Bioscience
SKU: 5148
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 5148

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Citations (105)

COA / SDS

Highly potent PORCN inhibitor

Key Product Details

Description:	Highly potent PORCN inhibitor
Chemical Name:	4-(2-Methyl-4-pyridinyl)-N-[4-(3-pyridinyl)phenyl]benzeneacetamide
Purity:	≥99% (HPLC)
Molecular Weight:	379.45

View Full Technical Details for Wnt-C59

Technical Data
Citations / Reviews (1)

Biological Activity for Wnt-C59

Wnt-C59 is a highly potent inhibitor of the MBOAT (membrane-bound O-acyltranferase) family member, Porcupine (PORCN) (IC₅₀ = 74 pM), that mediates WNT palmitoylation and secretion. Wnt-C59 potently inhibits the processing of both canonical (1, 2, 3a, 6, 7b, 8a, 9a, 9b, 10) and non-canonical (4, 5a, 11, 16) Wnt subtypes. Wnt-C59 blocks progression of mammary tumors in MMTV-WNT1 transgenic mice and downregulates Wnt/β-catenin target genes. Wnt-C59-treated tumors show a decrease in β-catenin, CyclinD1 and c-Myc. Wnt-C59 induces cardiomyocyte differentiation from human iPSCs following culture with CHIR 99021 (Cat. No. 4423). Wnt-C59 efficiently induces neural differentiation of CTIP2⁺/COUP-TF1^- cells from PSCs in culture. When grafted into the cortex of adult mice, Wnt-C59-treated cells develop abundant axonal fiber extensions toward the spinal cord. The compound has also been used in protocols to generate β cells from human PSCs. Cell permeable and orally bioavailable.

Compound Libraries for Wnt-C59

Wnt-C59 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for Wnt-C59

M. Wt	379.45
Formula	C₂₅H₂₁N₃O
Storage	Store at -20°C
Purity	≥99% (HPLC)
CAS Number	1243243-89-1
PubChem ID	57519544
InChi Key	KHZOJCQBHJUJFY-UHFFFAOYSA-N
Smiles	O=C(CC3=CC=C(C4=CC=NC(C)=C4)C=C3)NC1=CC=C(C2=CC=CN=C2)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Wnt-C59

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	7.59	20
	ethanol	7.59	20

Preparing Stock Solutions for Wnt-C59

The following data is based on the product molecular weight 379.45.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	13.18 mL	65.88 mL	131.77 mL
1 mM	2.64 mL	13.18 mL	26.35 mL
2 mM	1.32 mL	6.59 mL	13.18 mL
10 mM	0.26 mL	1.32 mL	2.64 mL

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Reconstitution Calculator

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References for Wnt-C59

References are publications that support the biological activity of the product.

Jiang Generation of pancreatic progenitors from human pluripotent stem cells by small molecules. Stem Cell Rep. 2021 PMID: 34450037
Motono WNT-C59, a small-molecule WNT inhibitor, efficiently induces anterior cortex that includes cortical motor neurons from human pluripotent stem cells. Stem Cells Transl.Med. 2016 PMID: 26941358
Burridge Chemically defined generation of human cardiomyocytes. Nat.Methods 2014 PMID: 24930130
Youssef Adult interfollicular tumour-initiating cells are reprogrammed into an embryonic hair follicle progenitor-like fate during basal cell carcinoma initiation. Nat. Cell Biol. 2012 PMID: 23178882
Wend WNT10B/β-catenin signalling induces HMGA2 and proliferation in metastatic triple-negative breast cancer. EMBO Mol.Med. 2013 PMID: 23307470
Proffitt Pharmacological inhibition of the Wnt acyltransferase PORCN prevents growth of WNT-driven mammary cancer. Cancer Res. 2013 PMID: 23188502