Improving Your 10X Genomic Workflows Using the Pala Cell Sorter from Bio-Techne

Single cell sequencing methods have emerged as powerful tools for identification of heterogeneous cell types. In this application note, we compared single-nucleus RNA sequencing (snRNA-seq) data from dissociated nuclei using traditional methods compared to sorting nuclei using Bio-Techne’s Pala benchtop cell sorter. The sequencing data showed the Pala system was able to sort nuclei to successfully clear debris from brain and kidney dissociated tissue, recover more cells for downstream 10X genomic workflows, and displays cleaner sequencing data with higher gene recognition and characterization of diverse cell types. The results serve as a reference for researchers seeking to improve their genomic workflow and uncover results that may have been hidden using traditional methods.