Benefits of Targeted Protein Degradation With PROTAC® E-book from Bio-Techne & Wiley

This edition of expert insights highlights advancements in the vibrant field of targeted protein degradation with small molecule drugs that aim for ‘undruggable’ targets. This eBook also connects researchers to new technologies accelerating drug discovery with robust high throughput screens and quantitative protein assays like the automated Simple Western™ platform from Bio-Techne. 

What you will learn about:

• Design considerations for PROTAC^® development
• How fully automated westerns enable cutting-edge TPD research
• Validating targets for TPD using dTAG – a comprehensive workflow solution

• Automated profiling of PROTAC^®-induced cereblon neosubstrate degradation using Simple Western

For cells to maintain protein homeostasis, they make use of two pathways − the ubiquitin-proteasome system (UPS) and autophagy-lysosomal degradation. In the UPS, proteins are initially ‘tagged’ by multimers of the ubiquitin protein, followed by their breakdown by a large enzyme complex known as the proteasome. Autophagy sequesters proteins in double-membrane vesicles and delivers them to the lysosome for breakdown. New chemical biology tools are allowing the manipulation of these two cellular processes with the concept of targeted protein degradation (TPD). The advantages of TPD have resulted in a considerable degree of interest in both the pharmaceutical industry and academia due to its potential drug discovery capabilities.