beta-Catenin Antibody (CTNNB1/2030R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79753
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # CTNNB1/2030R
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant full-length human beta-Catenin protein (Uniprot: P35222 )
Localization
Cell Surface, Cytoplasm
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Theoretical MW
92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified by Protein A Column. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79888)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for beta-Catenin Antibody (CTNNB1/2030R)
Western Blot: beta-Catenin Antibody (CTNNB1/2030R) [NBP2-79753] - Western Blot Analysis of HeLa cell lysate using beta-Catenin Antibody (CTNNB1/2030R).
Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (CTNNB1/2030R) [NBP2-79753] - Immunofluorescence Analysis of HeLa cells labeling Beta-Catenin with beta-Catenin Antibody (CTNNB1/2030R) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot (Red)
Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/2030R) [NBP2-79753] - Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with beta-Catenin Antibody (CTNNB1/2030R).
Applications for beta-Catenin Antibody (CTNNB1/2030R)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: beta-Catenin
Additional beta-Catenin Products
Product Documents for beta-Catenin Antibody (CTNNB1/2030R)
Product Specific Notices for beta-Catenin Antibody (CTNNB1/2030R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...