CD40 Ligand/TNFSF5 Antibody (chi220) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12025
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Block/Neutralize, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # chi220
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for CD40 Ligand/TNFSF5 Antibody (chi220) - Chimeric - Azide and BSA Free
Immunogen
This antibody was generated by immunizing female BALB/c mice with a fusion protein containing extracellular domain of human CD40 Ligand/TNFSF5 fused to the hinge CH2 and CH3 domains of a murine IgG2b antibody.
Specificity
This antibody binds the CD40 Ligand/TNFSF5 and blocks the humoral immune responses to T cell dependent antigens in a dose dependent manner.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for CD40 Ligand/TNFSF5 Antibody (chi220) - Chimeric - Azide and BSA Free
Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (chi220) - Chimeric [NBP3-12025] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of chi220 (NBP3-12025) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12025, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.
Applications for CD40 Ligand/TNFSF5 Antibody (chi220) - Chimeric - Azide and BSA Free
Application
Recommended Usage
Block/Neutralize
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: CD40 Ligand/TNFSF5
Alternate Names
CD154, CD40L, CD40LG, gp39, TNFSF5, TRAP
Gene Symbol
CD40LG
Additional CD40 Ligand/TNFSF5 Products
- All Products for CD40 Ligand/TNFSF5
- CD40 Ligand/TNFSF5 cDNA Clones
- CD40 Ligand/TNFSF5 ELISA Kits
- CD40 Ligand/TNFSF5 Luminex Assays
- CD40 Ligand/TNFSF5 Lysates
- CD40 Ligand/TNFSF5 Primary Antibodies
- CD40 Ligand/TNFSF5 Proteins and Enzymes
- CD40 Ligand/TNFSF5 Simple Plex
- CD40 Ligand/TNFSF5 Small Molecules and Peptides
Product Documents for CD40 Ligand/TNFSF5 Antibody (chi220) - Chimeric - Azide and BSA Free
Product Specific Notices for CD40 Ligand/TNFSF5 Antibody (chi220) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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